Departamento de Biotecnología Microbiana, Centro Nacional de Biotecnología (CNB-CSIC), Campus Cantoblanco, Universidad Autónoma de Madrid, Madrid 28049, Spain.
Mycologia. 2004 Mar-Apr;96(2):249-59.
The order Diatrypales (Ascomycota) contains one single family, the Diatrypaceae. To obtain insight in the phylogenetic relationships within this family, the complete sequences of the ITS region (ITS1, 5.8S rRNA gene and ITS2) of 53 isolates from the five main genera in the family (Diatrype, Diatrypella, Cryptosphaeria, Eutypa and Eutypella) were determined and aligned for phylogenetic reconstruction. Sequence analysis revealed the presence of tandem repeated motifs 11 nucleotides-long, placed in homologous positions along the ITS1 region. Parsimony analysis established the existence of nine monophyletic groups and one branch with a single isolate of Eutypella quaternata. The phylogenetic relationships established by parsimony analysis did not correlate well with classical taxonomic schemes. None of the five genera included in this study was found to be monophyletic. The genera Diatrype, Eutypa and Cryptosphaeria each were divided into two groups. Isolates of Diatrype flavovirens appeared in a clade separated from the one that grouped Diatrype disciformis and the rest of Diatrype species. The Eutypa strains appeared distributed into two clades, one grouping Eutypa lata and related species (Eutypa armeniacae, Eutypa laevata, Eutypa petrakii), and another with the remaining species of the genus. Eutypella (excluding Eutypella quaternaria) appeared as an unstable monophyletic group, which was lost when the sequence alignment was subjected to neighbor-joining analysis. The genus Diatrypella was not associated with any monophyletic group, suggesting that the multisporate asci character has appeared several times during the evolution of the group. Overall, our study suggests the need to revise many of the concepts usually applied to the classification of members of the family.
盘菌目（子囊菌门）仅包含一个科，即盘菌科。为了深入了解该科内的系统发育关系，本研究测定了来自该科五个主要属（地星属、拟盘菌属、 cryptoceras、eutypa 和 eutypella）的 53 个分离物的 ITS 区（ITS1、5.8S rRNA 基因和 ITS2）的全长序列，并进行了比对以进行系统发育重建。序列分析揭示了存在串联重复的 motif，长 11 个核苷酸，排列在 ITS1 区域的同源位置。简约分析确定了 9 个单系群和一个分支的存在，该分支仅包含一个 eutypella quaternata 的分离物。简约分析建立的系统发育关系与经典分类方案不相符。在所研究的五个属中，没有一个被发现是单系的。地星属、eutypa 和 cryptoceras 每个属都分为两个组。Diatrype flavovirens 的分离物出现在一个与分组 Diatrype disciformis 和其余 Diatrype 种的分支分开的分支中。Eutypa 菌株分布在两个分支中，一个分支聚集了 Eutypa lata 和相关种（Eutypa armeniacae、Eutypa laevata、Eutypa petrakii），另一个分支则是属内其余的种。Eutypella（不包括 Eutypella quaternaria）表现为一个不稳定的单系群，当对序列比对进行邻接法分析时，该群就会消失。拟盘菌属与任何单系群都没有关联，这表明多孢子的子囊特征在该属的进化过程中已经出现了多次。总的来说，本研究表明需要修订许多通常应用于该科成员分类的概念。
