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运用系统发育种识别来划定长喙壳属内的物种界限。

Using phylogenetic species recognition to delimit species boundaries within Lasiosphaeria.

机构信息

Botany Department, The Field Museum, 1400 S. Lake Shore Drive, Chicago, Illinois 60605-2496 and University of Illinois at Chicago, Department of Biological Sciences, Chicago, Illinois 60607-7060.

出版信息

Mycologia. 2004 Sep-Oct;96(5):1106-27.

PMID:21148930
Abstract

The genus Lasiosphaeria recently has been circumscribed more narrowly to include five mor-phospecies united by tomentose ascomata containing yellow centrum pigments. Species boundaries have not been established and phylogenetic relationships have not been clearly defined for these morphospecies. To delimit species boundaries and determine phylogenetic relationships among species, maximum parsimony, maximum likelihood and Bayesian analyses were conducted on sequence data from four nuclear genes, the ribosomal internal transcribed spacer (ITS) region, 28S large subunit (LSU) rDNA, β-tubulin and ribosomal polymerase II subunit 2 (RPB2). Representatives of L. glabrata, L. ovina, L. rugulosa and L. sorbina resolved as four highly supported monophyletic groups in almost all analyses and are recognized as well-defined species employing principles of genealogical concordance. These species delimitations are corroborated further by morphology. Representatives of L. lanuginosa were polyphyletic in almost all analyses. Although molecular analyses revealed that this morphospecies comprises several phylogenetic species, formal taxonomic recognition of these lineages is premature, so L. lanuginosa currently is treated as a morphological species complex. Complete species descriptions, including teleomorph, anamorph and culture characteristics, are given for L. glabrata, L. ovina, L. sorbina and the L. lanuginosa species complex along with detailed discussions of significant morphological characters used in recognizing species. These species are compared to five additional morphospecies that also may belong in the genus.

摘要

拉斯皮奥希亚菌属(Lasiosphaeria)最近被更狭义地定义为包括五个形态种,这些形态种的特征是具有绒毛状的子囊壳,其中包含黄色中心色素。尚未确定物种界限,也未明确界定这些形态种的系统发育关系。为了划定物种界限并确定种间的系统发育关系,对来自四个核基因(核糖体内部转录间隔区(ITS)区域、28S 大亚基(LSU)rDNA、β-微管蛋白和核糖体聚合酶 II 亚基 2(RPB2))的序列数据进行了最大简约法、最大似然法和贝叶斯分析。L. glabrata、L. ovina、L. rugulosa 和 L. sorbina 的代表在几乎所有分析中都分为四个高度支持的单系群,被认为是根据系统发育一致性原则定义明确的物种。这些物种的划分进一步得到形态学的支持。L. lanuginosa 的代表在几乎所有分析中都是多系的。尽管分子分析表明,这个形态种包含几个系统发育种,但这些谱系的正式分类识别还不成熟,因此 L. lanuginosa 目前被视为一个形态种复合体。对 L. glabrata、L. ovina、L. sorbina 和 L. lanuginosa 物种复合体进行了完整的种描述,包括有性型、无性型和培养特征,并详细讨论了用于识别物种的重要形态特征。将这些物种与另外五个可能属于该属的形态种进行了比较。

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