Universität Tübingen, Lehrstuhl Spezielle Botanik und Mykologie, Auf der Morgenstelle 1, D-72076 Tübingen, Germany.
Mycologia. 2004 Nov-Dec;96(6):1209-17.
Cellular interactions between the ascomycete Cymadothea trifolii and Trifolium repens (white clover) were analyzed using high-pressure freezing and freeze substitution. Cymadothea trifolii, a biotrophic leaf pathogen, forms a unique structure within its own hyphae, presumably for nutrient uptake from its host. This structure, called an interaction apparatus, consists of long, thin, often net-like cisternae surrounded by a membrane continuous with the fungal plasma membrane. The plant plasmalemma opposite the interaction apparatus invaginates to produce a host bubble. The interaction apparatus and host bubble are apoplastic and are linked by a tube with an electron dense sheath that may channel nutrients from the host to the pathogen. Within the tube, the cell walls of host and parasite appear altered. The interaction apparatus and host bubble may be analogous to haustoria in other obligately biotrophic fungi while the electron dense sheath of the tube may be equivalent to the haustorial neckband.
采用高压冷冻和冷冻替代技术分析了外囊菌属 Cymadothea trifolii 和三叶草（白车轴草）之间的细胞相互作用。Cymadothea trifolii 是一种生物营养性叶病原菌，在其自身菌丝内形成一种独特的结构，可能是为了从宿主中吸收营养。这种结构称为相互作用装置，由长而细的、常呈网状的潴泡体组成，被与真菌质膜连续的膜包围。与相互作用装置相对的植物质膜向内凹陷，形成宿主泡。相互作用装置和宿主泡是质外体的，通过一个具有电子致密鞘的管连接，该管可能将养分从宿主输送到病原体。在管内，宿主和寄生虫的细胞壁似乎发生了改变。相互作用装置和宿主泡可能类似于其他专性生物营养真菌中的吸器，而管的电子致密鞘可能相当于吸器颈带。
