Universität Tübingen, Lehrstuhl Spezielle Botanik und Mykologie, Auf der Morgenstelle 1, D-72076, Tübingen, Germany.
Mycologia. 2004 Sep-Oct;96(5):960-7.
Cellular interaction of the basidiomycete Tuberculina persicina with the haploid stages of two rusts Puccinia silvatica and Tranzschelia pruni-spinosae was analyzed by serial-section electron microscopy of chemically fixed samples and samples subjected to high-pressure freezing and freeze substitution. Tuberculina persicina is a contact parasite, forming neither haustoria nor other intracellular structures. However, at contact areas between T. persicina and its hosts, distinct interfungal interactions are present. At the beginning, a hyphal cell of T. persicina invades the host cell wall with a protuberance and the cell walls of both protuberance and host cell dissolve at the point of contact. Thus, the plasma membranes of the two organisms contact and fuse to form a pore that enlarges to a final diameter of approximately 1 μm. The membrane of the fusion pore is continuous with the plasma membranes of both cells, and Tuberculina nuclei and other organelles are transferred to the rust cells. Phylogenetic and functional aspects of this curious basidiomycetous interfungal interaction are discussed, and a hypothesis of the evolutionary derivation of the Tuberculina mycoparasitism from a sexual interaction is presented.
担子菌 Tuberculina persicina 与两种锈菌 Puccinia silvatica 和 Tranzschelia pruni-spinosae 的单倍体阶段的细胞相互作用通过化学固定样品和高压冷冻及冷冻替代处理的连续切片电子显微镜分析进行了研究。Tuberculina persicina 是一种接触寄生虫，既不形成吸器，也不形成其他细胞内结构。然而，在 T. persicina 与其宿主之间的接触区域存在明显的真菌间相互作用。最初，T. persicina 的一个菌丝细胞以突起的形式侵入宿主细胞壁，并且突起和宿主细胞壁在接触点溶解。因此，两个生物体的质膜接触并融合形成一个孔，最终直径约为 1 μm。融合孔的膜与两个细胞的质膜连续，Tuberculina 核和其他细胞器被转移到锈菌细胞中。讨论了这种奇特的担子菌间真菌相互作用的系统发育和功能方面，并提出了 Tuberculina 菌寄生从有性相互作用进化而来的假说。
