Kobayashi Yukihiro, Miyazawa Maki, Kamei Asuka, Abe Keiko, Kojima Takashi
Chemistry Division, Kanagawa Prefectural Institute of Public Health, Chigasaki, Kanagawa, Japan.
Biosci Biotechnol Biochem. 2010;74(12):2385-95. doi: 10.1271/bbb.100392. Epub 2010 Dec 7.
To determine the effects of mulberry (Morus alba L.) leaves on hyperlipidemia, we performed gene expression profiling of the liver. Rats were fed a high-fat diet and administered mulberry leaves for 7 weeks. Plasma triglyceride and non-esterified fatty acid levels were significantly lower in the rats treated with mulberry leaves as compared with the untreated rats. DNA microarray analysis revealed that mulberry leaves upregulated expression of the genes involved in α-, β- and ω-oxidation of fatty acids, mainly related to the peroxisome proliferator-activated receptor signaling pathway, and downregulated the genes involved in lipogenesis. Furthermore, treatment with mulberry leaves upregulated expression of the genes involved in the response to oxidative stress. These results indicate that consumption of fatty acids and inhibition of lipogenesis are responsible for the reduction in plasma lipids caused by mulberry administration. In addition, mulberry treatment maintains the body's oxidative state at a low level despite enhancing fatty acid oxidation.
为了确定桑叶（Morus alba L.）对高脂血症的影响，我们对肝脏进行了基因表达谱分析。给大鼠喂食高脂饮食并给予桑叶7周。与未处理的大鼠相比，用桑叶处理的大鼠血浆甘油三酯和非酯化脂肪酸水平显著降低。DNA微阵列分析显示，桑叶上调了参与脂肪酸α-、β-和ω-氧化的基因表达，主要与过氧化物酶体增殖物激活受体信号通路相关，并下调了参与脂肪生成的基因。此外，用桑叶处理上调了参与氧化应激反应的基因表达。这些结果表明，脂肪酸的消耗和脂肪生成的抑制是桑叶给药导致血浆脂质降低的原因。此外，尽管增强了脂肪酸氧化，但桑叶处理仍将机体的氧化状态维持在较低水平。
