Departamento de Nutrição e Dietética, Universidade Federal do Rio de Janeiro, Brazil.
Ann Nutr Metab. 2010;57(3-4):242-50. doi: 10.1159/000322187. Epub 2010 Dec 11.
BACKGROUND/AIMS: To determine the influence of the Pro12Ala polymorphism of the PPARγ2 gene and the dietary lipid intake on energy metabolism and nutritional outcomes in obese women after an acute fat load or following a low-calorie diet for 10 weeks.
Sixty obese women (aged 30-46 years) participated in the study and were assigned to 2 groups depending on the genotype: Pro12Pro and Pro12Ala/Ala12Ala carriers. At baseline and after 2 nutritional (short- or long-term) interventions, measurement of anthropometrical and body composition (bioelectrical impedance) variables, dietary assessments, energy metabolism (indirect calorimetry) measurements as well as biochemical and molecular (PPARγ2 genotype) analyses were performed. All women received a high-fat test meal to determine the postprandial metabolism (short term) and an energy-restricted diet for 10 weeks (long term).
The frequencies of the Pro12Pro and Pro12Ala/Ala12Ala genotypes were 83.33 and 16.67%, respectively, and reached Hardy-Weinberg equilibrium. Interestingly, the postprandial energy expenditure after the fat load was higher in subjects carrying the Ala allele. At baseline, the habitual monounsaturated fatty acid (MUFA) intake inversely correlated with fat oxidation and body mass index in the obese Pro12Ala/Ala12Ala carriers, while a lower PUFA intake (%) in the long-term trial was associated with an increase in the respiratory quotient only in Ala carriers but not in the Pro12Pro genotyped group.
The Pro12Ala polymorphism in the PPARγ2 gene influenced energy metabolism in the assayed short- and long-term situations since the response to both nutritional interventions differed according to the genotype. The results suggest that fat oxidation and energy expenditure may be lower in Pro12Pro carriers compared to Pro12Ala/Ala12Ala genotypes, while in obese women with Pro12Ala/Ala12Ala polymorphisms in the PPARγ2 gene fat oxidation was negatively correlated with the MUFA and PUFA (%) intake.
背景/目的：确定 PPARγ2 基因 Pro12Ala 多态性和膳食脂质摄入对肥胖女性在急性脂肪负荷后或在 10 周低热量饮食后的能量代谢和营养结局的影响。
60 名肥胖女性（年龄 30-46 岁）参与了这项研究，并根据基因型分为 2 组：Pro12Pro 和 Pro12Ala/Ala12Ala 携带者。在基线和 2 种营养（短期或长期）干预后，测量了人体测量学和身体成分（生物电阻抗）变量、饮食评估、能量代谢（间接测热法）测量以及生化和分子（PPARγ2 基因型）分析。所有女性都接受了高脂肪测试餐以确定餐后代谢（短期）和 10 周的能量限制饮食（长期）。
Pro12Pro 和 Pro12Ala/Ala12Ala 基因型的频率分别为 83.33%和 16.67%，并达到 Hardy-Weinberg 平衡。有趣的是，在携带 Ala 等位基因的受试者中，脂肪负荷后的餐后能量消耗更高。在基线时，肥胖的 Pro12Ala/Ala12Ala 携带者中，习惯性单不饱和脂肪酸（MUFA）摄入与脂肪氧化和体重指数呈负相关，而在长期试验中，较低的多不饱和脂肪酸（PUFA）摄入（%）仅与 Ala 携带者的呼吸商增加相关，但与 Pro12Pro 基因型组无关。
PPARγ2 基因的 Pro12Ala 多态性影响了所检测的短期和长期情况下的能量代谢，因为对这两种营养干预的反应根据基因型而不同。结果表明，与 Pro12Pro 携带者相比，Pro12Ala/Ala12Ala 基因型的脂肪氧化和能量消耗可能较低，而在 PPARγ2 基因中存在 Pro12Ala/Ala12Ala 多态性的肥胖女性中，脂肪氧化与 MUFA 和 PUFA（%）摄入呈负相关。
