• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

禽成红细胞增多症病毒转化的细胞中珠蛋白基因表达的转录后抑制

Post-transcriptional suppression of globin gene expression in cells transformed by avian erythroblastosis virus.

作者信息

Therwath A, Scherrer K

出版信息

Proc Natl Acad Sci U S A. 1978 Aug;75(8):3776-80. doi: 10.1073/pnas.75.8.3776.

DOI:10.1073/pnas.75.8.3776
PMID:211506
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC392869/
Abstract

Cells transformed by avian erythroblastosis virus were grown in vitro for up to 5 months. After a few days in culture, synthesis of hemoglobin was undetectable and could not be induced by dimethyl sulfoxide. As shown by globin cDNA hybridization to nuclear and cytoplasmic RNA carried to Crot values of 10(5) moles of nucleotide per liter X sec, globin genes in these cells are transcribed into pre-mRNA, but no trace of globin mRNA appears in the cytoplasm. The implications of this observation for schemes of post-transcriptional regulations and viral transformation are discussed.

摘要

由禽成红细胞增多症病毒转化的细胞在体外培养长达5个月。培养几天后，无法检测到血红蛋白的合成，且二甲基亚砜也无法诱导其合成。通过珠蛋白cDNA与核RNA和细胞质RNA杂交至10⁵摩尔核苷酸每升×秒的Crot值表明，这些细胞中的珠蛋白基因被转录成前体mRNA，但细胞质中未出现珠蛋白mRNA的踪迹。本文讨论了这一观察结果对转录后调控和病毒转化机制的意义。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/35a6/392869/014a5b7474b9/pnas00020-0248-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/35a6/392869/e172c3291233/pnas00020-0248-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/35a6/392869/d50605614eb2/pnas00020-0248-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/35a6/392869/014a5b7474b9/pnas00020-0248-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/35a6/392869/e172c3291233/pnas00020-0248-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/35a6/392869/d50605614eb2/pnas00020-0248-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/35a6/392869/014a5b7474b9/pnas00020-0248-c.jpg

相似文献

1
Post-transcriptional suppression of globin gene expression in cells transformed by avian erythroblastosis virus.禽成红细胞增多症病毒转化的细胞中珠蛋白基因表达的转录后抑制
Proc Natl Acad Sci U S A. 1978 Aug;75(8):3776-80. doi: 10.1073/pnas.75.8.3776.
2
In vitro transformation by avian erythroblastosis virus recruits cells of erythroid lineage with varying degree of differentiation.禽成红细胞增多症病毒介导的体外转化会募集不同分化程度的红系谱系细胞。
Int J Cancer. 1982 May 15;29(5):555-8. doi: 10.1002/ijc.2910290511.
3
Modification of the methylation pattern in the vicinity of the chicken globin genes in avian erythroblastosis virus transformed cells.禽成红细胞增多症病毒转化细胞中鸡珠蛋白基因附近甲基化模式的改变。
Nucleic Acids Res. 1981 Apr 24;9(8):1841-51. doi: 10.1093/nar/9.8.1841.
4
Altered globin gene transcription pattern and the presence of a 7-8 kb alpha A globin gene transcript in avian erythroblastosis virus-transformed cells.禽成红细胞增多症病毒转化细胞中珠蛋白基因转录模式的改变及一种7 - 8 kbαA珠蛋白基因转录本的存在。
EMBO J. 1984 Mar;3(3):491-5. doi: 10.1002/j.1460-2075.1984.tb01835.x.
5
Transcription of the alpha globin gene domain in normal and AEV-transformed chicken erythroblasts: mapping of giant globin-specific RNA including embryonic and adult genes.正常和禽成红细胞增多症病毒（AEV）转化的鸡成红细胞中α珠蛋白基因结构域的转录：包括胚胎和成人基因的巨大珠蛋白特异性RNA的定位
Mol Gen Genet. 1987 Sep;209(2):210-20. doi: 10.1007/BF00329645.
6
Virus gene expression in rat cells transformed by avian myelocytomatosis virus strain MC29 and avian erythroblastosis virus.禽成髓细胞瘤病毒MC29株和禽成红细胞增多症病毒转化的大鼠细胞中的病毒基因表达
J Gen Virol. 1983 Jan;64 (Pt 1):83-94. doi: 10.1099/0022-1317-64-1-83.
7
Activation of cellular genes by avian RNA tumor viruses.禽RNA肿瘤病毒对细胞基因的激活作用。
Proc Natl Acad Sci U S A. 1980 Sep;77(9):5351-4. doi: 10.1073/pnas.77.9.5351.
8
Characterization of the oncogene (erb) of avian erythroblastosis virus and its cellular progenitor.禽成红细胞增多症病毒癌基因（erb）及其细胞起源的特征分析。
J Virol. 1981 May;38(2):409-19. doi: 10.1128/JVI.38.2.409-419.1981.
9
[Transcription of globin gene in erythroid cells transformed by Rauscher virus].[劳舍尔病毒转化的红系细胞中珠蛋白基因的转录]
Biokhimiia. 1982 Feb;47(2):312-6.
10
Avian erythroblastosis virus produces two mRNA's.禽成红细胞增多症病毒产生两种信使核糖核酸。
J Virol. 1980 Dec;36(3):676-83. doi: 10.1128/JVI.36.3.676-683.1980.

引用本文的文献

1
Genetic test for involvement of intervening sequences in transport of nuclear RNA.关于间隔序列参与核RNA转运的基因检测。
Mol Cell Biol. 1982 Dec;2(12):1550-7. doi: 10.1128/mcb.2.12.1550-1557.1982.
2
Variation of expression of histocompatibility antigens on tumor cells: absence of H-2Kk-gene products from a gross-virus-induced leukemia in BALB.K.肿瘤细胞上组织相容性抗原表达的变化：BALB.K品系中一种粗病毒诱导的白血病缺乏H-2Kk基因产物
Immunogenetics. 1981;14(3-4):323-39. doi: 10.1007/BF00342201.
3
Genetic variation in rates of antipyrine metabolite formation: a study in uninduced twins.

本文引用的文献

1
REGULATION OF THE INITIATION OF CHICK EMBRYO HEMOGLOBIN SYNTHESIS.鸡胚血红蛋白合成起始的调控
J Mol Biol. 1965 Jun;12:331-41. doi: 10.1016/s0022-2836(65)80257-1.
2
Messenger RNA in avian erythroblasts at the transcriptional and translational levels and the problem of regulation in animal cells.禽类成红细胞中转录和翻译水平的信使核糖核酸以及动物细胞中的调控问题。
J Cell Physiol. 1968 Oct;72(2):Suppl 1:181+. doi: 10.1002/jcp.1040720413.
3
Quantitative differences in the content of the histone f2c between chicken erythrocytes and erythroblasts.
安替比林代谢产物形成速率的基因变异：一项针对未诱导双胞胎的研究。
Proc Natl Acad Sci U S A. 1981 Aug;78(8):5193-6. doi: 10.1073/pnas.78.8.5193.
4
RNA metabolism in isolated nuclei: processing and transport of immunoglobulin light chain sequences.分离细胞核中的RNA代谢：免疫球蛋白轻链序列的加工与转运
Nucleic Acids Res. 1981 Sep 25;9(18):4767-81. doi: 10.1093/nar/9.18.4767.
5
Altered globin gene transcription pattern and the presence of a 7-8 kb alpha A globin gene transcript in avian erythroblastosis virus-transformed cells.禽成红细胞增多症病毒转化细胞中珠蛋白基因转录模式的改变及一种7 - 8 kbαA珠蛋白基因转录本的存在。
EMBO J. 1984 Mar;3(3):491-5. doi: 10.1002/j.1460-2075.1984.tb01835.x.
6
Modification of the methylation pattern in the vicinity of the chicken globin genes in avian erythroblastosis virus transformed cells.禽成红细胞增多症病毒转化细胞中鸡珠蛋白基因附近甲基化模式的改变。
Nucleic Acids Res. 1981 Apr 24;9(8):1841-51. doi: 10.1093/nar/9.8.1841.
7
Subcellular localization of RNAs in transfected cells: role of sequences at the 5' terminus.RNA在转染细胞中的亚细胞定位：5'端序列的作用
Nucleic Acids Res. 1984 Nov 26;12(22):8363-80. doi: 10.1093/nar/12.22.8363.
8
Relationship between the transport from isolated nuclei of two abundant cytoplasmic messengers and the source of a messenger RNA transport factor.两种丰富的细胞质信使从分离细胞核的转运与信使核糖核酸转运因子来源之间的关系
Mol Biol Rep. 1984 Jan;9(4):227-30. doi: 10.1007/BF00775352.
9
No evidence for post-transcriptional control of albumin and alpha-fetoprotein gene expression in developing rat liver neoplasia.在发育中的大鼠肝脏肿瘤形成过程中，没有证据表明白蛋白和甲胎蛋白基因表达存在转录后调控。
Nucleic Acids Res. 1982 Mar 25;10(6):1895-911. doi: 10.1093/nar/10.6.1895.
10
AEV-transformed erythroleukemia cell induced differentiation: expression of specific cell membrane antigenic molecules.禽成红细胞增多症病毒转化的红白血病细胞诱导分化：特异性细胞膜抗原分子的表达
Arch Virol. 1987;93(3-4):213-22. doi: 10.1007/BF01310975.
鸡红细胞和成红细胞中组蛋白f2c含量的定量差异。
Exp Cell Res. 1972 Jul;73(1):13-6. doi: 10.1016/0014-4827(72)90095-x.
4
Principles and practices of nucleic acid hybridization.核酸杂交的原理与实践
Prog Nucleic Acid Res Mol Biol. 1971;11:259-301. doi: 10.1016/s0079-6603(08)60330-x.
5
The second Sir Hans Krebs Lecture. Informosomes.第二届汉斯·克雷布斯爵士讲座。信息体
Eur J Biochem. 1969 Aug;10(1):20-35. doi: 10.1111/j.1432-1033.1969.tb00651.x.
6
Repeated sequences in DNA. Hundreds of thousands of copies of DNA sequences have been incorporated into the genomes of higher organisms.DNA中的重复序列。数以十万计的DNA序列拷贝已被纳入高等生物的基因组中。
Science. 1968 Aug 9;161(3841):529-40. doi: 10.1126/science.161.3841.529.
7
Purification and further properties of single-strand-specific nuclease from Aspergillus oryzae.米曲霉单链特异性核酸酶的纯化及其他特性
Eur J Biochem. 1973 Feb 15;33(1):192-200. doi: 10.1111/j.1432-1033.1973.tb02669.x.
8
Cultivation of rat bone marrow. II. Long-term erythrocyte production in vitro.大鼠骨髓培养。II. 体外长期红细胞生成
J Natl Cancer Inst. 1972 Oct;49(4):1071-5.
9
Synthesis and processing of nuclear precursor-messenger RNA in avian erythroblasts and HeLa cells.鸟类成红细胞和海拉细胞中核内前体信使RNA的合成与加工
Proc Natl Acad Sci U S A. 1974 Dec;71(12):5009-13. doi: 10.1073/pnas.71.12.5009.
10
Globin messenger RNA synthesis and processing during haemoglobin induction in Friend cells. II. Evidence for post-transcriptional control in clone 707.弗氏细胞血红蛋白诱导过程中的珠蛋白信使核糖核酸合成与加工。II. 克隆707中转录后调控的证据。
Cell Differ. 1974 Jun;3(1):23-30. doi: 10.1016/0045-6039(74)90037-2.