分离细胞核中的RNA代谢：免疫球蛋白轻链序列的加工与转运

RNA metabolism in isolated nuclei: processing and transport of immunoglobulin light chain sequences.

作者信息

Otegui C, Patterson R J

出版信息

Nucleic Acids Res. 1981 Sep 25;9(18):4767-81. doi: 10.1093/nar/9.18.4767.

DOI:10.1093/nar/9.18.4767

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC327474/

Abstract

Transport of prelabeled RNA from isolated myeloma nuclei is studied using conditions that permit RNA synthesis. Cytosol and spermidine are not required to maintain nuclear stability and inhibited RNA release. Omission of ATP or GTP decreased release 25 to 40%. The stimulatory effect of ATP or GTP is not due to hydrolysis of the triphosphates by the nuclear envelope NTPase, since addition of quercetin (an inhibitor of this NTPase) has no effect on the quantity of RNA released. The size distribution and percentage of poly A-containing species released from nuclei incubated with or without ATP or the other rNTPs are identical. Hybridization analysis of nuclear RNA before the transport assay revealed mature and precursor k light chain mRNA sequences. Following the transport assay, a significant fraction of k mRNA precursors is chased into mature k mRNA which is found both in nuclear-retained and released RNA.

摘要

利用允许RNA合成的条件，研究了从分离的骨髓瘤细胞核中运输预先标记的RNA的过程。维持核稳定性和抑制RNA释放不需要胞质溶胶和亚精胺。省略ATP或GTP会使释放量降低25%至40%。ATP或GTP的刺激作用并非由于核膜NTPase对三磷酸的水解，因为添加槲皮素（这种NTPase的抑制剂）对释放的RNA量没有影响。在有或没有ATP或其他rNTP的情况下孵育的细胞核中释放的含聚A物种的大小分布和百分比是相同的。运输分析之前对核RNA的杂交分析揭示了成熟和前体κ轻链mRNA序列。运输分析之后，相当一部分κmRNA前体被追踪到成熟的κmRNA中，在核保留和释放的RNA中都能发现这种成熟的κmRNA。

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本文引用的文献

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Biochemistry. 1980 Jun 10;19(12):2719-23. doi: 10.1021/bi00553a028.

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Transcription of adenoviral genetic information in isolated nuclei. Characterization of viral RNA sequences synthesized in vitro.腺病毒遗传信息在分离细胞核中的转录。体外合成的病毒RNA序列的特征分析。

J Biol Chem. 1980 Mar 10;255(5):2097-108.

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Effects of thioacetamide treatment on nuclear envelope nucleoside triphosphatase activity and transport of RNA from rat liver nuclei.硫代乙酰胺处理对大鼠肝细胞核膜核苷三磷酸酶活性及RNA从细胞核转运的影响。

Cancer Res. 1980 Jan;40(1):75-9.

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