Stewart N P, de Vos A J, Shiels I
Queensland Department of Primary Industries, Animal Research Institute, Wacol, Queensland, Australia.
Trop Anim Health Prod. 1990 May;22(2):109-15. doi: 10.1007/BF02239835.
Fifty splenectomised calves naturally infected with Theileria buffeli were treated with primaquine phosphate (ICI, UK) and halofuginone lactate (Hoechst, Australia) either separately or in combination. Infections in treated calves were monitored for up to 26 weeks by examining Giemsa stained peripheral blood films for piroplasms and by an immunofluorescent antibody test. When used alone neither of the drugs eliminated infection. The most successful results were obtained when two treatments of halofuginone lactate, at a rate of 1 mg kg-1 body weight and six treatments of primaquine phosphate, at a rate of 2 mg kg-1 body weight, were administered concurrently. No theilerial relapses were observed in 14 of 16 calves so treated, and no antibody to T. buffeli was detected in these calves beyond the twelfth week after treatment. The results have application in the preparation of Theileria-free calves for use in the production of living vaccines against babesiosis and anaplasmosis.
五十头自然感染水牛泰勒虫的脾切除小牛，分别或联合使用磷酸伯氨喹（英国帝国化学工业公司生产）和乳酸盐卤夫酮（澳大利亚赫斯特公司生产）进行治疗。通过检查吉姆萨染色的外周血涂片以寻找梨形虫，并通过免疫荧光抗体试验，对治疗后的小牛感染情况进行长达26周的监测。单独使用这两种药物均无法消除感染。当以1毫克/千克体重的剂量进行两次乳酸盐卤夫酮治疗，并以2毫克/千克体重的剂量进行六次磷酸伯氨喹治疗同时给药时，取得了最成功的结果。接受如此治疗的16头小牛中有14头未观察到泰勒虫复发，并且在治疗后第12周之后，这些小牛中未检测到针对水牛泰勒虫的抗体。这些结果可应用于制备无泰勒虫的小牛，用于生产抗巴贝斯虫病和无形体病的活疫苗。
