Cubillas Raul, Kintner Katherine, Phillips Frances, Karandikar Nitin J, Thiele Dwain L, Brown Geri R
Division of Digestive and Liver Diseases, Department of Internal Medicine, Southwestern Medical Center at Dallas, University of Texas at Dallas, Dallas, TX 75390-9151, USA.
Hepat Res Treat. 2010;2010:429243. doi: 10.1155/2010/429243. Epub 2010 Aug 3.
The present studies assessed the level of tumor necrosis factor receptor (TNFR) expression in peripheral blood mononuclear cells (PBMCs) subsets from patients with chronic HCV undergoing interferon α/ribavirin-based therapy (Ifn/R). Methods. TNFR family member mRNA expression was determined using quantitative real-time PCR assays (RTPCRs) in PBMC from 39 HCV+ patients and 21 control HCV- patients. Further subset analysis of HCV + patients (untreated (U), sustained virological responders (SVR), and nonresponders (NR)/relapsers (Rel)) PBMC was performed via staining with anti-CD123, anti-CD33, anti-TNFR1 or via RTPCR for TNFR1 mRNA. Results. A similar level of TNFR1 mRNA in PBMC from untreated HCV+ genotype 1 patients and controls was noted. TNFR1 and TNFR2 mRNA levels in PBMC from HCV+ patients with SVR were statistically different than levels in HCV(-) patients. A significant difference was noted between the peak values of TNFR1 of the CD123+ PBMC isolated from SVR and the NR/Rel. Conclusion. Upregulation of TNFR1 expression, occurring in a specific subset of CD123+ dendritic cells, appeared in HCV+ patients with SVR.
本研究评估了接受基于干扰素α/利巴韦林治疗（Ifn/R）的慢性丙型肝炎患者外周血单个核细胞（PBMC）亚群中肿瘤坏死因子受体（TNFR）的表达水平。方法。采用定量实时PCR检测法（RTPCR）测定39例HCV阳性患者和21例HCV阴性对照患者外周血单个核细胞中TNFR家族成员mRNA的表达。通过用抗CD123、抗CD33、抗TNFR1染色或通过RTPCR检测TNFR1 mRNA，对HCV阳性患者（未治疗（U）、持续病毒学应答者（SVR）和无应答者（NR）/复发者（Rel））的外周血单个核细胞进行进一步亚群分析。结果。未治疗的HCV基因1型阳性患者和对照患者外周血单个核细胞中TNFR1 mRNA水平相似。SVR的HCV阳性患者外周血单个核细胞中TNFR1和TNFR2 mRNA水平与HCV阴性患者相比有统计学差异。从SVR和NR/Rel分离的CD123 +外周血单个核细胞中TNFR1峰值之间存在显著差异。结论。TNFR1表达上调出现在SVR的HCV阳性患者中，发生于CD123 +树突状细胞的特定亚群。
