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中华绒螯蟹表达序列标签中的16个多态性简单序列重复标记。

Sixteen polymorphic simple sequence repeat markers from expressed sequence tags of the Chinese Mitten Crab Eriocheir sinensis.

作者信息

Gao Xiang-Gang, Li Hong-Jun, Li Yun-Feng, Sui Li-Jun, Zhu Bao, Liang Yu, Liu Wei-Dong, He Chong-Bo

机构信息

Liaoning Key Laboratory of Marine Fishery Molecular Biology, Liaoning Ocean and Fishery Science Institute, 50 Heishijiao St, Dalian, Liaoning 116023, China; E-Mail:

出版信息

Int J Mol Sci. 2010 Aug 24;11(8):3035-8. doi: 10.3390/ijms11083035.

DOI:10.3390/ijms11083035
PMID:21152289
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2996744/
Abstract

The Chinese mitten crab (Eriocheir sinensis) is an economically important aquaculture species in China. In this study, we developed and evaluated simple sequence repeat markers from expressed sequence tags of E. sinensis. Among the 40 wild E. sinensis individuals tested, 16 loci were polymorphic. The number of alleles per locus ranged from two to ten. The observed heterozygosity ranged from 0.0667 to 0.9667, whereas the expected heterozygosity ranged from 0.0661 to 0.9051. These markers have the potential for use in genetic studies of population structure and intraspecific variation in E. sinensis.

摘要

中华绒螯蟹是中国一种具有重要经济价值的水产养殖物种。在本研究中，我们从中华绒螯蟹的表达序列标签中开发并评估了简单序列重复标记。在所测试的40只野生中华绒螯蟹个体中，16个位点具有多态性。每个位点的等位基因数量从2到10不等。观察到的杂合度范围为0.0667至0.9667，而预期杂合度范围为0.0661至0.9051。这些标记物有潜力用于中华绒螯蟹种群结构和种内变异的遗传研究。

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