从中华绒螯蟹表达序列标签中鉴定和表征三十个新型微卫星DNA标记。
Identification and characterization of thirty novel microsatellite DNA markers from the Chinese mitten crab Eriocheir sinensis expressed sequence tags.
作者信息
Li Jingjing, Geng Xuyun, Chen Limei, Sun Jinsheng
机构信息
Tianjin Key Laboratory for Animal and Plant Resistance, College of Life Sciences, Tianjin Normal University, 393 Binshuixi Rd, Xiqing District, Tianjin, 300387, China.
Tianjin Diseases Prevention and Control Center of Aquatic Animals, 442 Jiefangnan Rd, Hexi District, Tianjin, 300221, China.
出版信息
BMC Res Notes. 2016 Feb 17;9:105. doi: 10.1186/s13104-016-1909-6.
BACKGROUND
The Chinese mitten crab Eriocheir sinensis is an economically important decapod crustacean in China. Despite a widespread distribution and production in China, the resources of E. sinensis have experienced a dramatic decline in the past decades. Here we describe a new set of novel polymorphic microsatellite loci to facilitate the investigation of genetic structure and artificial breeding.
RESULTS
In this study, a set of 30 novel polymorphic microsatellite markers for E. sinensis was developed from EST databases. The number of alleles per locus ranged from three to twenty. The observed and expected heterozygosities ranged from 0.047 to 0.932 and from 0.047 to 0.935, respectively.
CONCLUSIONS
These informative microsatellite markers will be useful in studies of genetics, genomics and marker-assisted selection breeding in E. sinensis.
背景
中华绒螯蟹是中国一种具有重要经济价值的十足目甲壳动物。尽管在中国分布广泛且产量可观，但在过去几十年里，中华绒螯蟹的资源量急剧下降。在此，我们描述了一组新的新型多态微卫星位点，以促进对其遗传结构和人工养殖的研究。
结果
在本研究中，从EST数据库开发了一组30个用于中华绒螯蟹的新型多态微卫星标记。每个位点的等位基因数从3到20不等。观察到的杂合度和期望杂合度分别在0.047至0.932和0.047至0.935之间。
结论
这些信息丰富的微卫星标记将有助于中华绒螯蟹的遗传学、基因组学及标记辅助选择育种研究。
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