Jiang Peng, Dai Weixing, Yan Shikai, Chen Zhongliang, Xu Ruilin, Ding Jianmi, Xiang Li, Wang Shuping, Liu Runhui, Zhang Weidong
School of Pharmacy, Second Military Medical University, No. 325 Guohe Road, Shanghai 200433, P.R. China.
Mol Biosyst. 2011 Mar;7(3):824-31. doi: 10.1039/c0mb00110d. Epub 2010 Dec 10.
A metabolomic method using reversed-phase liquid chromatography/quadrupole time-of-flight mass spectrometry (LC-Q-TOF-MS) was developed to obtain a systematic view of the development and progression of myocardial infarction (MI). By combining with partial least squares discriminant analysis (PLS-DA), 16 biomarkers in rat urine were identified and eight of them were related to the pathway of energy metabolism. Among the regulated pathways, the citric acid cycle related network was acutely perturbed. The metabolomic results not only supplied a systematic view of the development and progression of MI but also provided the theoretical basis for the prevention or treatment of MI. The developed method was also used to analyze the therapeutic effects of a traditional Chinese medicine (TCM) named Shexiang Baoxin Pill (SBP), a widely used anti-MI medicine in clinics. The results showed that SBP administration could provide satisfactory effects on MI through partially regulating the perturbed pathway of energy metabolism.
开发了一种使用反相液相色谱/四极杆飞行时间质谱（LC-Q-TOF-MS）的代谢组学方法，以全面了解心肌梗死（MI）的发生和发展过程。通过结合偏最小二乘判别分析（PLS-DA），鉴定出大鼠尿液中的16种生物标志物，其中8种与能量代谢途径相关。在受调控的途径中，柠檬酸循环相关网络受到急性干扰。代谢组学结果不仅提供了MI发生和发展的全面视图，还为MI的预防或治疗提供了理论依据。所开发的方法还用于分析一种名为麝香保心丸（SBP）的传统中药的治疗效果，SBP是临床上广泛使用的抗MI药物。结果表明，给予SBP可通过部分调节能量代谢紊乱的途径对MI提供满意的疗效。
