Research Institute for Electronic Science, Hokkaido University, N20W10 Kita-Ku, Sapporo, 001-0020, Japan.
Photochem Photobiol Sci. 2011 Apr;10(4):461-3. doi: 10.1039/c0pp00271b. Epub 2010 Dec 13.
The time-dependent nature of the complexes of IR806 dye and other similar near-infrared cyanine dyes in human serum albumin (HSA) have been studied by employing absorption, emission and time-correlated single photon counting techniques. The complex formation of IR806 with HSA modifies the native structure of IR806, resulting in time-dependent changes in its optical properties. The modification process of the dye and its complex formation with HSA is very slow and takes about 90 min. The process of the formation of the new complex is irreversible and totally controlled by the initial complex of IR806 and HSA. As far as we know, this new property of cyanine dyes has not been reported before. These properties are very important for near-infrared fluorescence imaging.
采用吸收、发射和时间相关单光子计数技术研究了 IR806 染料和其他类似近红外花菁染料与人血清白蛋白（HSA）之间的复合物的时间依赖性。IR806 与 HSA 的复合物形成修饰了 IR806 的天然结构，导致其光学性质随时间发生变化。染料的修饰过程及其与 HSA 的复合物形成非常缓慢，大约需要 90 分钟。新配合物的形成过程是不可逆的，完全受 IR806 和 HSA 的初始配合物控制。据我们所知，以前尚未报道过花菁染料的这种新特性。这些性质对于近红外荧光成像是非常重要的。
