Cunha Fabiane, Gómez Daniel R Sosa, da Silva Jose J, Alexandre Talita M, Moscardi Flávio
Pós-Graduação em Entomologia, UFPR, Embrapa Soja, Laboratório de Patologia de Insetos Caixa Postal 231, 86001-970 Londrina, PR, Brasil.
An Acad Bras Cienc. 2010 Dec;82(4):1127-36. doi: 10.1590/s0001-37652010000400029.
Chlosyne lacinia saundersii is one of the most important pests of sunflower and it is the main target of insecticides applications. Larvae were collected in Londrina (PR), Santa Maria (RS), Dourados (MS), Ribeirão Preto (SP), Brasília (DF), Barreiras (BA), Uberaba (MG) and Vilhena (RO). Genomic DNA was extracted and amplified with ten-mer primers, which produced 101 loci. The size of the RAPD amplicons ranged from 180 to 2564 bp. Polymorphism among populations ranged from 31% to 67%, with the highest polymorphisms of 57% and 67% being detected in Uberaba and Vilhena populations, respectively. Populations with the highest similarity determined with Dice coefficient were from Ribeirão Preto and Barreiras, while insects from Londrina showed the highest similarity among them. Gene flow of C. lacinia saundersii 1.1 was lower than those previously observed for the noctuid Anticarsia gemmatalis Hübner, suggesting that C. lacinia saundersii populations are more isolated than the ones of this noctuid. Through the Analysis of Molecular Variance (AMOVA), RAPD variance was 33.64% among geographical populations and 66.36% within populations. These results suggest that populations of C. lacinia saundersii are genetically structured.
桑氏黄蛱蝶是向日葵最重要的害虫之一，也是杀虫剂施用的主要目标。幼虫采集于隆德里纳（巴拉那州）、圣玛丽亚（南里奥格兰德州）、多拉杜斯（南马托格罗索州）、里贝朗普雷图（圣保罗州）、巴西利亚（联邦区）、巴雷拉斯（巴伊亚州）、乌贝拉巴（米纳斯吉拉斯州）和维莱纳（朗多尼亚州）。提取基因组DNA并用十聚体引物进行扩增，产生了101个位点。随机扩增多态性DNA（RAPD）扩增片段大小在180至2564碱基对之间。种群间的多态性在31%至67%之间，乌贝拉巴和维莱纳种群分别检测到最高多态性，为57%和67%。用戴斯系数确定的相似度最高的种群来自里贝朗普雷图和巴雷拉斯，而隆德里纳的昆虫在它们之中相似度最高。桑氏黄蛱蝶的基因流为1.1，低于之前观察到的夜蛾科害虫棉铃虫，这表明桑氏黄蛱蝶种群比这种夜蛾的种群更隔离。通过分子方差分析（AMOVA），地理种群间的RAPD方差为33.64%，种群内为66.36%。这些结果表明桑氏黄蛱蝶种群具有遗传结构。
