Department of Chemistry, Hong Kong Baptist University, Kowloon, Hong Kong SAR, China.
Anal Bioanal Chem. 2011 Apr;399(10):3431-9. doi: 10.1007/s00216-010-4463-4. Epub 2010 Dec 9.
Prolonged intake of aristolochic acid (AA) has been shown to be associated with the development of certain renal disorders. Renal tubular atrophy and interstitial fibrosis are the early symptoms of AA nephropathy. The symptoms were observed in rats that were dosed with AA at a dosage of 10 mg/kg/day for 1 month. Apart from the renal tubular atrophy and interstitial fibrosis, AA-DNA adducts were detected in the rat kidney tissue. Differentiated proteins were identified in the kidney tissues from proteomics investigations. The upregulated proteins identified included ornithine aminotransferase, sorbitol dehydrogenase, actin, aspartoacylase, 3-hydroxyisobutyrate dehydrogenase, and peroxiredoxin-1. Downregulated proteins such as ATP synthase subunit β, glutamate dehydrogenase 1, regucalcin, glutamate-cysteine ligase regulatory subunit, dihydropteridine reductase, hydroxyacyl-coenzyme A dehydrogenase, voltage-dependent anion-selective channel protein 1, prohibitin, and adenylate kinase isoenzyme 4 were also identified. Several identified protein markers were found to have biological and medical significance.
长期摄入马兜铃酸（AA）已被证明与某些肾脏疾病的发展有关。肾小管萎缩和间质纤维化是马兜铃酸肾病的早期症状。在给大鼠每天用 AA 剂量为 10mg/kg 处理 1 个月后，观察到了这些症状。除了肾小管萎缩和间质纤维化之外，还在大鼠肾脏组织中检测到 AA-DNA 加合物。通过蛋白质组学研究鉴定出了分化的蛋白质。鉴定出的上调蛋白包括鸟氨酸转氨酶、山梨醇脱氢酶、肌动蛋白、天冬氨酸酰氨酶、3-羟基异丁酸脱氢酶和过氧化物酶 1。下调的蛋白如 ATP 合酶亚基β、谷氨酸脱氢酶 1、钙调节蛋白、谷氨酸-半胱氨酸连接酶调节亚基、二氢喋呤还原酶、羟酰基辅酶 A 脱氢酶、电压依赖性阴离子选择通道蛋白 1、抑制素和腺苷酸激酶同工酶 4 也被鉴定出来。一些鉴定出的蛋白质标记物具有生物学和医学意义。
