Ecole Polytechnique Fédérale de Lausanne, ENAC Laboratory for Environmental Biotechnology, Station 6, CH-1015, Lausanne, Switzerland.
Appl Microbiol Biotechnol. 2011 Apr;90(1):361-8. doi: 10.1007/s00253-010-3042-0. Epub 2010 Dec 9.
The presence of chloroethene dechlorination activity as well as several bacterial genera containing mainly organohalide-respiring members was investigated in 34 environmental samples from 18 different sites. Cultures inoculated with these environmental samples on tetrachloroethene and amended weekly with a seven organic electron donor mixture resulted in 11 enrichments with cis-DCE, ten with VC, and 11 with ethene as dechlorination end product, and only two where no dechlorination was observed. "Dehalococcoides" spp. and Desulfitobacterium spp. were detected in the majority of the environmental samples independently of the dechlorination end product formed. The concomitant presence of Dehalococcoides spp. and Desulfitobacterium spp. in the majority of the enrichments suggested that chloroethene dechlorination was probably the result of catalysis by at least two organohalide-respiring genera either in parallel or by stepwise catalysis. A more detailed study of one enrichment on cis-DCE suggested that in this culture Desulfitobacterium spp. as well as Dehalococcoides spp. dechlorinated cis-DCE whereas dechlorination of VC was only catalyzed by the latter.
研究了来自 18 个不同地点的 34 个环境样本中是否存在氯乙烯脱氯活性以及主要包含有机卤化物呼吸成员的几个细菌属。用这些环境样本接种培养物，在每周添加七有机电子供体混合物的情况下，用四氯乙烯进行接种培养，结果得到 11 个以顺式-二氯乙烯、10 个以 VC、11 个以乙烯为脱氯终产物的富集物，只有 2 个没有观察到脱氯。在大多数环境样本中，无论形成何种脱氯终产物，都检测到了“Dehalococcoides” spp. 和脱硫菌属 spp。大多数富集物中同时存在 Dehalococcoides spp. 和脱硫菌属 spp. ，这表明氯乙烯脱氯可能是至少两种有机卤化物呼吸属通过平行或逐步催化作用的结果。对顺式-二氯乙烯的一个富集物的更详细研究表明，在该培养物中，脱硫菌属 spp. 以及 Dehalococcoides spp. 脱氯顺式-二氯乙烯，而 VC 的脱氯仅由后者催化。
