College of Animal Science and Technology, Sichuan Agricultural University, Ya'an, Sichuan, People's Republic of China.
Mol Cell Biochem. 2011 Apr;350(1-2):39-46. doi: 10.1007/s11010-010-0680-6. Epub 2010 Dec 9.
Studies have shown that not only does palmitic acid promote triglyceride (TG) accumulation, but it also affects cell viability in in vitro steatosis models. However, to what degree these effects are mediated by steatosis in goose primary hepatocytes is unknown. In this study, the effects of palmitic acid on the lipid metabolism homeostasis pathway and on apoptosis were determined. The authors measured the mRNA levels of genes involved in TG synthesis, lipid deposition, fatty acid oxidation and the assembly and secretion of VLDL-TG in goose primary hepatocytes. The results indicated that palmitic acid can significantly reduce the activity of goose hepatocytes, and that palmitic acid had a significant effect on TG accumulation; however, with increasing palmitic acid concentrations, the extracellular TG and extracellular VLDL concentration gradually decreased. With increasing palmitic acid concentrations, the gene expression levels of DGAT1, DGAT2, PPARα, CPT-1, FoxO1 and MTTP (which regulate hepatic TG synthesis, fatty acid oxidation and the assembly and secretion of VLDL-TGs) first increased and then decreased; the change in PLIN gene expression was palmitic acid dose-dependent, similar to the regulatory mode of intracellular TG accumulation. In conclusion, this study clearly shows that palmitic acid can promote TG accumulation and induce apoptosis in goose primary hepatocytes, and this effect may be related to the lipid metabolism pathway.
研究表明，棕榈酸不仅促进甘油三酯（TG）的积累，而且还影响体外脂肪变性模型中的细胞活力。然而，这些作用在鹅原代肝细胞中脂肪变性的程度尚不清楚。在这项研究中，作者确定了棕榈酸对脂质代谢稳态途径和细胞凋亡的影响。作者测量了参与 TG 合成、脂质沉积、脂肪酸氧化以及 VLDL-TG 组装和分泌的基因在鹅原代肝细胞中的 mRNA 水平。结果表明，棕榈酸可显著降低鹅肝细胞的活性，并且棕榈酸对 TG 积累有显著影响；然而，随着棕榈酸浓度的增加，细胞外 TG 和细胞外 VLDL 浓度逐渐降低。随着棕榈酸浓度的增加，调节肝 TG 合成、脂肪酸氧化和 VLDL-TG 组装和分泌的 DGAT1、DGAT2、PPARα、CPT-1、FoxO1 和 MTTP 的基因表达水平先增加后减少；PLIN 基因表达的变化与棕榈酸剂量呈依赖性，类似于细胞内 TG 积累的调节模式。总之，本研究清楚地表明，棕榈酸可促进鹅原代肝细胞中 TG 的积累并诱导细胞凋亡，这种作用可能与脂质代谢途径有关。
