Institute of Animal Genetics and Breeding, College of Animal Science and Technology, Sichuan Agricultural University, Ya'an, Sichuan 625014, P.R.China.
Comp Biochem Physiol A Mol Integr Physiol. 2011 Jun;159(2):113-8. doi: 10.1016/j.cbpa.2011.01.019. Epub 2011 Feb 2.
Studies have shown linoleate could not only promote cell viability but also affect lipid metabolism in mammals. However, to what degree these effects are mediated by steatosis in goose primary hepatocytes is unknown. In this study, the effect of linoleate on the lipid metabolic homeostasis pathway was determined. We measured the mRNA levels of genes involved in triglyceride synthesis, lipid deposition, β-oxidation, and assembly and secretion of VLDL-TGs in goose (Anser cygnoides) primary hepatocytes. Linoleate significantly increased goose hepatocyte viability, and linoleate at 0.125 mM, 0.25 mM, 0.5 mM and 1.0 mM all showed a significant effect on TG accumulation. However, with increasing linoleate concentrations, the extracellular TG concentration and extracellular VLDL gradually decreased. DGAT1, DGAT2, PPARα, PPARγ, FoxO1, MTP, PLIN and CPT-1 mRNA was detected by real-time PCR. With increasing linoleate concentrations, the changes in DGAT1, DGAT2, PPARα and CPT-1 gene expression, which regulates hepatic TG synthesis and fatty acid oxidation, first increased and then decreased. Additionally, FoxO1 and MTP gene expression was reduced with increasing linoleate concentrations, and the change in PLIN gene expression was increased at all concentrations, similar to the regulation of intracellular TG accumulation. In conclusion, linoleate regulated TG accumulation and increased hepatocyte viability. The data suggest that linoleate does promote goose hepatocyte viability and steatosis, which may up-regulate TG synthesis-relevant gene expression, suppress assembly and secretion of VLDL-TGs, and increase fatty acid oxidation properly to function of goose primary hepatocytes.
研究表明，亚油酸不仅可以促进细胞活力，还可以影响哺乳动物的脂质代谢。然而，这些作用在鹅原代肝细胞中的脂肪变性中程度如何尚不清楚。在这项研究中，确定了亚油酸对脂质代谢稳态途径的影响。我们测量了参与甘油三酯合成、脂质沉积、β-氧化以及 VLDL-TG 组装和分泌的基因在鹅（Anser cygnoides）原代肝细胞中的 mRNA 水平。亚油酸显著增加了鹅肝细胞活力，并且在 0.125 mM、0.25 mM、0.5 mM 和 1.0 mM 浓度的亚油酸都对 TG 积累表现出显著影响。然而，随着亚油酸浓度的增加，细胞外 TG 浓度和细胞外 VLDL 逐渐降低。通过实时 PCR 检测 DGAT1、DGAT2、PPARα、PPARγ、FoxO1、MTP、PLIN 和 CPT-1 的 mRNA。随着亚油酸浓度的增加，调节肝 TG 合成和脂肪酸氧化的 DGAT1、DGAT2、PPARα 和 CPT-1 基因表达的变化先增加后减少。此外，FoxO1 和 MTP 基因表达随着亚油酸浓度的增加而减少，PLIN 基因表达的变化在所有浓度下均增加，与细胞内 TG 积累的调节相似。总之，亚油酸调节 TG 积累并增加肝细胞活力。数据表明，亚油酸确实促进了鹅肝细胞的活力和脂肪变性，这可能会上调与 TG 合成相关的基因表达，抑制 VLDL-TG 的组装和分泌，并适当增加脂肪酸氧化，以维持鹅原代肝细胞的功能。
