College of Environment and Resources, Jilin University, Changchun, Jilin, 130026, People's Republic of China.
Environ Monit Assess. 2011 Oct;181(1-4):43-9. doi: 10.1007/s10661-010-1811-y. Epub 2010 Dec 9.
A multi-media bio-PRB reactor was designed to treat groundwater contaminated with petroleum hydrocarbons. After a 208-day bioremediation, combined with the total petroleum hydrocarbons content in the groundwater flowed through the reactor, microbiological characteristics of the PRB reactor including microbes immobilized and its dehydrogenase activity were investigated. TPH was significantly reduced by as much as 65% in the back of the second media layer, whereas in the third layer, the TPH content reached lower than 1 mg l⁻¹. For microbes immobilized on the media, the variations with depth in different media were significantly the same and the regularity was obvious in the forepart of the media, which increased with depth at first and then reduced gradually, while in the back-end, the microbes almost did not have any variations with depth but decreased with the distance. The dehydrogenase activity varied from 2.98 to 16.16 mg TF L⁻¹ h⁻¹ and its distribution illustrated a similar trend with numbers of microbial cell, therefore, the noticeable correlation was found between them.
设计了一种多媒体生物 PRB 反应器来处理受石油烃污染的地下水。经过 208 天的生物修复，结合通过反应器的地下水中的总石油烃含量，研究了 PRB 反应器的微生物特性，包括固定化微生物及其脱氢酶活性。在后两层的第二个介质层中，TPH 显著降低了 65%，而在第三层中，TPH 含量达到了 1mg/L 以下。对于固定在介质上的微生物，不同介质中深度的变化非常相似，在前部介质中规律明显，最初随深度增加，然后逐渐减少，而在后部，微生物几乎没有任何深度变化，但随距离而减少。脱氢酶活性从 2.98 到 16.16mg TF/L/h 不等，其分布与微生物细胞数量的分布趋势相似，因此，发现它们之间存在显著相关性。
