Ural Division, Komi Science Center, Institute of Biology, Russian Academy of Sciences, Syktyvkar, Russia.
Biogerontology. 2011 Jun;12(3):211-26. doi: 10.1007/s10522-010-9311-6. Epub 2010 Dec 9.
The GADD45 protein family plays an important role in stress signaling and participates in the integration of cellular response to environmental and physiological factors. GADD45 proteins are involved in cell cycle control, DNA repair, apoptosis, cell survival and aging, and inflammatory response by complicated protein-protein interactions. In Drosophila melanogaster a single D-GADD45 ortholog (GG1086) has been described. Our data show that overexpression of the D-GADD45 gene in the nervous system leads to a significantly increase of Drosophila lifespan without a decrease in fecundity and locomotor activity. The lifespan extension effect is more pronounced in males than in females, which agrees with the sex-dependent expression of this gene. The longevity of D. melanogaster with D-GADD45 overexpression is apparently due to more efficient recognition and repair of DNA damage, as the DNA comet assay showed that the spontaneous DNA damage in the larva neuroblasts is reduced with statistical significance.
GADD45 蛋白家族在应激信号转导中发挥重要作用，并参与细胞对环境和生理因素的反应整合。GADD45 蛋白通过复杂的蛋白-蛋白相互作用参与细胞周期调控、DNA 修复、细胞凋亡、细胞存活和衰老以及炎症反应。在黑腹果蝇中，已经描述了一个单一的 D-GADD45 直系同源物（GG1086）。我们的数据表明，在神经系统中过表达 D-GADD45 基因会导致果蝇寿命显著延长，而不会降低生育力和运动活性。这种寿命延长效应在雄性中比在雌性中更为明显，这与该基因的性别依赖性表达一致。D. melanogaster 中 D-GADD45 的过表达导致 DNA 损伤的更有效识别和修复，这一点从 DNA 彗星试验中可以看出，该试验显示幼虫神经母细胞中的自发 DNA 损伤显著减少。
