Mertani H C, Delehaye-Zervas M C, Martini J F, Postel-Vinay M C, Morel G
CNRS URA 1454, Neuroendocrinology, Lyon-Sud School of Medicine, Oullins.
Endocrine. 1995 Feb;3(2):135-42. doi: 10.1007/BF02990065.
In order to identify GH target cells in human tissues, we investigated the celiuiar distribution of human GH receptor (GHR) messenger RNA. This was performed byin situ hybridization and Northern blot hybridization using complementary radioactive DNA probe encoding part of the extracellular domain of the GHR. Several tests were carried out to validate the detection of gene expression. Our results demonstrate that the GHR gene is expressed in all human tissues studied, including liver, muscle, kidney, heart, skin, thymus, adipose tissue, placenta, testis, ovary and mammary gland. The quantification of thein situ hybridization signal by densitometric analysis showed a greater expression of GHR transcript in liver, muscle, kidney, heart, and skin epidermis, and a reduced expression in thymus, mammary gland, testis, ovary, and dermis/ hypodermis. These results confirm the widespread distribution of the GHR gene expression in human tissues and identify the cells that might be responsive to GH.
为了在人体组织中识别生长激素（GH）的靶细胞，我们研究了人类生长激素受体（GHR）信使核糖核酸的细胞分布。这是通过使用编码GHR细胞外结构域部分的互补放射性DNA探针进行原位杂交和Northern印迹杂交来完成的。进行了多项测试以验证基因表达的检测。我们的结果表明，GHR基因在所研究的所有人体组织中均有表达，包括肝脏、肌肉、肾脏、心脏、皮肤、胸腺、脂肪组织、胎盘、睾丸、卵巢和乳腺。通过密度分析对原位杂交信号进行定量显示，GHR转录本在肝脏、肌肉、肾脏、心脏和皮肤表皮中表达较高，而在胸腺、乳腺、睾丸、卵巢以及真皮/皮下组织中表达较低。这些结果证实了GHR基因表达在人体组织中的广泛分布，并确定了可能对GH有反应的细胞。
