Expression of the growth hormone receptor gene in human digestive tissue.

We have examined the forms and the distribution of the messenger ribonucleic acids (mRNAs) encoding the GH receptor (GHR) in human digestive tissues. GHR mRNAs were identified and characterized by Northern blot, dot blot, and in situ hybridization analyses, using complementary DNAs coding for the extracellular part of the human liver GHR. Amplification using the polymerase chain reaction was also used, as tissues express low levels of GHR mRNAs. Our results demonstrate that the GHR gene is expressed in human liver, pancreas, esophagus, stomach, small intestine, and colon. A single 4.5-kilobase mRNA form, which probably encodes the full-length membrane receptor, was detected. GHR mRNA was visualized by in situ hybridization in hepatocytes, exocrine pancreas, and some islet cells; signal was also present in the mucosa of the digestive tract. No specific GH-binding-protein mRNA was found, suggesting that in man, the soluble form of the receptor is generated through proteolytic cleavage of the membrane receptor.