Delehaye-Zervas M C, Mertani H, Martini J F, Nihoul-Feketé C, Morel G, Postel-Vinay M C
INSERM U-344, Paris, France.
J Clin Endocrinol Metab. 1994 Jun;78(6):1473-80. doi: 10.1210/jcem.78.6.8200952.
We have examined the forms and the distribution of the messenger ribonucleic acids (mRNAs) encoding the GH receptor (GHR) in human digestive tissues. GHR mRNAs were identified and characterized by Northern blot, dot blot, and in situ hybridization analyses, using complementary DNAs coding for the extracellular part of the human liver GHR. Amplification using the polymerase chain reaction was also used, as tissues express low levels of GHR mRNAs. Our results demonstrate that the GHR gene is expressed in human liver, pancreas, esophagus, stomach, small intestine, and colon. A single 4.5-kilobase mRNA form, which probably encodes the full-length membrane receptor, was detected. GHR mRNA was visualized by in situ hybridization in hepatocytes, exocrine pancreas, and some islet cells; signal was also present in the mucosa of the digestive tract. No specific GH-binding-protein mRNA was found, suggesting that in man, the soluble form of the receptor is generated through proteolytic cleavage of the membrane receptor.
我们已经研究了人类消化组织中编码生长激素受体（GHR）的信使核糖核酸（mRNA）的形式和分布。使用编码人肝GHR细胞外部分的互补DNA，通过Northern印迹、斑点印迹和原位杂交分析对GHR mRNA进行了鉴定和表征。由于组织中GHR mRNA表达水平较低，还使用了聚合酶链反应进行扩增。我们的结果表明，GHR基因在人肝、胰腺、食管、胃、小肠和结肠中表达。检测到一种单一的4.5千碱基mRNA形式，它可能编码全长膜受体。通过原位杂交在肝细胞、外分泌胰腺和一些胰岛细胞中观察到GHR mRNA；在消化道黏膜中也存在信号。未发现特异性生长激素结合蛋白mRNA，这表明在人类中，受体的可溶性形式是通过膜受体的蛋白水解裂解产生的。
