Misawa Y, Tojo A, Shibuya M
Institute of Medical Science, University of Tokyo, Japan.
Biochem Biophys Res Commun. 1990 Jul 16;170(1):39-45. doi: 10.1016/0006-291x(90)91237-m.
We isolated two genes that are highly expressed in Friend erythroleukemia. The first one, expressed at an extremely high level in a late-stage tumorigenic cell line F5-5, was found to be a species of intracisternal A-particle. Most of those genomes in F5-5 DNA were not amplified nor rearranged compared with those in the normal cell DNA, suggesting transcriptional activation. Another late-stage cell line T3K showed similar results. The second gene, highly expressed in early-stage cells but weaker in late-stage cells, was identified as one of the heme-synthesizing enzymes, uroporphyrinogen decarboxylase. Usefulness of these two genes for the analysis of erythroid transformation and differentiation will be discussed.
我们分离出了在Friend红白血病中高表达的两个基因。第一个基因在晚期致瘤细胞系F5-5中极高水平表达，被发现是一种核内A颗粒。与正常细胞DNA中的基因组相比，F5-5 DNA中的大多数基因组未扩增也未重排，提示转录激活。另一个晚期细胞系T3K也显示出类似结果。第二个基因在早期细胞中高表达但在晚期细胞中表达较弱，被鉴定为血红素合成酶之一，尿卟啉原脱羧酶。将讨论这两个基因在分析红细胞转化和分化中的用途。
