Institute of Human Nutrition and Food Science, Christian-Albrechts-University Kiel, Hermann-Rodewald-Str. 6, 24118, Kiel, Germany,
Cytotechnology. 2011 Jan;63(1):89-97. doi: 10.1007/s10616-010-9324-7. Epub 2010 Dec 10.
The present investigation aimed to compare fetal calf serum (FCS) and Tween 40 with the commonly employed tetrahydrofuran (THF) with respect to cytotoxicity, stability of the solubilized carotenoids, and uptake and accumulation of the xanthophylls astaxanthin (AX) and canthaxanthin (CX) in cultured human liver cells (HepG2). Incubation of HepG2 cells for 24 h with THF (≥1.25%) or FCS (≥11.25%) with or without AX (≥25 μmol/L) or CX (≥25 μmol/L) did not affect cell viability. Tween 40 (0.25-1.25% in medium) reduced cell viability by 75-99%. The stabilities of AX and CX in cell-free RPMI 1640 medium for ≤24 h were higher when delivered with THF instead of FCS. The dose- and time-dependent accumulations of AX and CX (1-10 μmol/L) in HepG2 cells were higher when carotenoids were delivered with FCS compared to THF. In conclusion, FCS and THF, but not Tween 40, were suitable solvent systems for the delivery of AX and CX to HepG2 cells. In our experiments FCS was superior with regard to the uptake and accumulation of both carotenoids.
本研究旨在比较胎牛血清（FCS）和吐温 40 与常用的四氢呋喃（THF）在细胞毒性、已溶解类胡萝卜素的稳定性以及类胡萝卜素虾青素（AX）和角黄素（CX）在培养的人肝细胞（HepG2）中的摄取和积累方面的差异。用 THF（≥1.25%）或 FCS（≥11.25%）孵育 HepG2 细胞 24 小时，无论是否含有 AX（≥25 μmol/L）或 CX（≥25 μmol/L），均不会影响细胞活力。吐温 40（培养基中 0.25-1.25%）可使细胞活力降低 75-99%。在不含细胞的 RPMI 1640 培养基中，AX 和 CX 的稳定性在≤24 小时内，用 THF 传递时比用 FCS 传递时更高。AX 和 CX（1-10 μmol/L）在 HepG2 细胞中的剂量和时间依赖性积累，当用 FCS 传递时比用 THF 传递时更高。总之，FCS 和 THF 可作为向 HepG2 细胞传递 AX 和 CX 的合适溶剂系统，而 Tween 40 则不适合。在我们的实验中，FCS 在两种类胡萝卜素的摄取和积累方面都具有优势。
