Department of Biology, Eastern New Mexico University, Roosevelt Hall, Room 101, Station 33, Portales, NM 88130, USA.
Curr Microbiol. 2011 Apr;62(4):1139-46. doi: 10.1007/s00284-010-9839-3. Epub 2010 Dec 12.
Antimicrobial agent usage is common in animal agriculture for therapeutic and prophylactic purposes. Selective pressure exerted by these antimicrobials on soil bacteria could result in the selection of strains that are resistant due to chromosomal- or plasmid-derived genetic components. Multiple antimicrobial resistances in Escherichia coli and the direct relationship between antimicrobial agent use over time has been extensively studied, yet the relationship between the age of an animal agriculture environment such as a dairy farm and antibiotic resistance remains unclear. Therefore, we tested the hypothesis that antimicrobial-resistance profiles of E. coli isolated from dairy farm topsoil correlate with dairy farm age. E. coli isolated from eleven dairy farms of varying ages within Roosevelt County, NM were used for MIC determinations to chloramphenicol, nalidixic acid, penicillin, tetracycline, ampicillin, amoxicillin/clavulanic acid, gentamicin, trimethoprim/sulfamethoxazole, cefotaxime, and ciprofloxacin. The minimum inhibitory concentration values of four antibiotics ranged 0.75 to >256 μg/ml, 1 to >256 μg/ml, 12 to >256 μg/ml, and 0.75 to >256 μg/ml for chloramphenicol, nalidixic acid, penicillin, and tetracycline, respectively. The study did not show a direct relationship between antibiotic resistance and the age of dairy farms.
抗菌剂在动物农业中被广泛用于治疗和预防目的。这些抗菌剂对土壤细菌施加的选择压力可能导致由于染色体或质粒衍生的遗传成分而产生耐药菌株。大肠杆菌中存在多种抗生素耐药性，并且抗生素使用时间与抗生素耐药性之间存在直接关系，这已经得到了广泛研究，然而动物农业环境（如奶牛场）的年龄与抗生素耐药性之间的关系尚不清楚。因此，我们检验了这样一个假设，即从新墨西哥州罗斯福县不同年龄的 11 个奶牛场的表层土壤中分离出的大肠杆菌的抗生素耐药谱与奶牛场的年龄有关。使用从新墨西哥州罗斯福县的 11 个不同年龄的奶牛场中分离出的大肠杆菌来进行 MIC 测定，以确定对氯霉素、萘啶酸、青霉素、四环素、氨苄西林、阿莫西林/克拉维酸、庆大霉素、复方磺胺甲噁唑、头孢噻肟和环丙沙星的最小抑菌浓度值。氯霉素、萘啶酸、青霉素和四环素的最小抑菌浓度值范围分别为 0.75 至>256μg/ml、1 至>256μg/ml、12 至>256μg/ml 和 0.75 至>256μg/ml。研究并未显示抗生素耐药性与奶牛场的年龄之间存在直接关系。
