Instituto de Biología Molecular y Celular de Plantas (Consejo Superior de Investigaciones Científicas - Universidad Politécnica de Valencia), Avenida de Naranjos, 46022 Valencia, Spain.
Arch Virol. 2011 Mar;156(3):529-33. doi: 10.1007/s00705-010-0867-x. Epub 2010 Dec 14.
Potato spindle tuber viroid (PSTVd) contains an element of tertiary structure -loop E- also present in eukaryotic 5S rRNA. The ribosomal protein L5 and transcription factor IIIA (TFIIIA) from Arabidopsis thaliana bind 5S rRNA in vitro and in vivo, mediating different functions that include nucleocytoplasmic transport and transcription activation, respectively. We show that A. thaliana L5 and TFIIIA also bind PSTVd (+) RNA in vitro with the same affinity as they bind 5S rRNA, whereas the affinity for a chloroplastic viroid is significantly lower. These two proteins might participate in the synthesis and delivery of PSTVd RNA in vivo.
马铃薯纺锤块茎类病毒（PSTVd）含有一个三级结构环 E 元件，该元件也存在于真核生物 5S rRNA 中。拟南芥核糖体蛋白 L5 和转录因子 IIIA（TFIIIA）在体外和体内与 5S rRNA 结合，分别介导包括核质转运和转录激活在内的不同功能。我们表明，拟南芥 L5 和 TFIIIA 也以与结合 5S rRNA 相同的亲和力在体外结合 PSTVd（+）RNA，而与叶绿体类病毒的亲和力则显著降低。这两种蛋白可能参与 PSTVd RNA 在体内的合成和递呈。
