Department of Biology, College of Sciences, Shiraz University, Iran.
Interdiscip Sci. 2010 Dec;2(4):367-72. doi: 10.1007/s12539-010-0040-y. Epub 2010 Dec 12.
Infection by human papillomavirus type 16 (HPV-16) is the cause of 50% or more of cervical cancers in women. The E7 oncoprotein of HPV-16 has long been known as a potent immortalizing and transforming agent. We used different servers like PseAAC, MHC_binding, MHC_II_binding and Expasy for the present computational prediction. The results for T cell epitopes showed that B1501, A0203, A0201, A0202, A6801 and DRB0405 alleles had lower IC50 than other alleles. We also predicted several peptides with the best binding affinities for alleles of the most frequent MHC class I and II alleles of the various ethnic groups living in the different region of Iran. Two peptides (26-35) and (44-52) were predicted as B-cell epitopes. According to this analysis 1 N-glycosylation site, 2 PKC sites, 4 CK2 sites and 3 disulfide sites were predicted. Our computational study predicted that B cell epitope 1 was Casein kinase II phosphorylated (site No. 31) and glycosylated (site No. 29). Putative MHC-I epitopes 3 and 5 and MHC-II epitopes 19, 21 and 26 were predicted to be casein kinase II phosphorylated. MHC-II epitopes 19 and 21 was predicted to be glycosylated. T cell epitopes 1, 13, 16 and 24 were demonstrated to be kinase C phosphorylated. The result of this analysis for Iranian HPV-16 E7 also indicated that 21.43%, 18.37% and 60.20% of the protein were in the α-helix, extended strand and random coil respectively.
人乳头瘤病毒 16 型（HPV-16）感染是女性宫颈癌的 50%或更多的原因。HPV-16 的 E7 癌蛋白长期以来一直被认为是一种有效的永生化和转化剂。我们使用不同的服务器，如 PseAAC、MHC_binding、MHC_II_binding 和 Expasy 进行目前的计算预测。T 细胞表位的结果表明，B1501、A0203、A0201、A0202、A6801 和 DRB0405 等位基因的 IC50 低于其他等位基因。我们还预测了一些与伊朗不同地区不同种族最常见的 MHC Ⅰ类和Ⅱ类等位基因具有最佳结合亲和力的肽。两个肽（26-35）和（44-52）被预测为 B 细胞表位。根据该分析，预测到 1 个 N-糖基化位点、2 个 PKC 位点、4 个 CK2 位点和 3 个二硫键。我们的计算研究预测 B 细胞表位 1 为酪蛋白激酶 II 磷酸化（位点编号 31）和糖基化（位点编号 29）。推定的 MHC-I 表位 3 和 5 以及 MHC-II 表位 19、21 和 26 被预测为酪蛋白激酶 II 磷酸化。MHC-II 表位 19 和 21 被预测为糖基化。T 细胞表位 1、13、16 和 24 被证明为蛋白激酶 C 磷酸化。对伊朗 HPV-16 E7 的分析结果还表明，该蛋白的α-螺旋、延伸链和无规卷曲部分分别占 21.43%、18.37%和 60.20%。
