Department of Biotechnology, Faculty of Advanced Sciences and Technologies, University of Isfahan, Isfahan, Iran.
Appl Biochem Biotechnol. 2019 Jan;187(1):90-100. doi: 10.1007/s12010-018-2804-5. Epub 2018 Jun 11.
One of the most dangerous human pathogens with high prevalence worldwide is Streptococcus pyogenes, which has major impacts on global morbidity and mortality. A major challenge for S. pyogenes vaccine development is the detection of epitopes that confer protection from infection by multiple S. pyogenes types. Our aim was to identify the most conserved and immunogenic antigens of S. pyogenes, which can be a potential candidate for vaccine design in the future. Eight important surface proteins were analyzed. Using different prediction servers, strongest epitopes were selected. They had the ability to stimulate the humoral and cell-mediated immune system. Molecular docking was performed for measuring free-binding energy of selected epitopes. Seven epitopes from three surface proteins were selected as potential candidates for vaccine development. Conservation of selected epitopes among different Streptococcus types was checked. Further in vitro and in vivo tests are required to validate the suitability of the epitopes for vaccine design.
全球流行率高的最危险的人类病原体之一是化脓性链球菌，它对全球发病率和死亡率有重大影响。化脓性链球菌疫苗开发的主要挑战是检测能够针对多种化脓性链球菌类型的感染提供保护的表位。我们的目的是确定化脓性链球菌中最保守和免疫原性最强的抗原，这些抗原可能成为未来疫苗设计的潜在候选物。分析了 8 种重要的表面蛋白。使用不同的预测服务器选择了最强的表位。它们具有刺激体液和细胞介导的免疫系统的能力。进行了分子对接以测量所选表位的自由结合能。从三种表面蛋白中选择了七个表位作为疫苗开发的潜在候选物。检查了所选表位在不同链球菌类型之间的保守性。需要进一步的体外和体内试验来验证表位用于疫苗设计的适宜性。
