Pinho Vanessa, Coelho Fernanda Matos, Menezes Gustavo Batista, Cara Denise Carmona
Department of Morphology, ICB, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil.
Methods Mol Biol. 2011;689:81-90. doi: 10.1007/978-1-60761-950-5_6.
The intravital microscopy is a valuable tool to capture images of cells in living organisms and to make studies of molecular determinants of leukocyte trafficking easier. Using this technique, we can directly visualize and measure each step of the leukocyte recruitment paradigm, including leukocyte rolling flux, rolling velocity, adhesion, and emigration. Thus, it is possible to understand the process involved in leukocyte homing as well as the cell recruitment to inflammatory tissues. Nowadays, two types of intravital microscopy are used routinely. The light microscopy is used to assess migration of intravascular cells in thin, tissues which must be sufficiently translucent. Epifluorescence microscopy allows the visualization of the microcirculation while permitting the distinction of leukocyte subpopulations in solid organs.
活体显微镜检查是一种宝贵的工具，可用于捕捉活生物体中细胞的图像，并使白细胞运输的分子决定因素的研究更加容易。使用这项技术，我们可以直接观察和测量白细胞募集模式的每一步，包括白细胞滚动通量、滚动速度、黏附和迁移。因此，有可能了解白细胞归巢以及细胞募集到炎症组织所涉及的过程。如今，两种类型的活体显微镜检查被常规使用。光学显微镜用于评估薄组织中血管内细胞的迁移，这些组织必须足够透明。落射荧光显微镜可以观察微循环，同时可以区分实体器官中的白细胞亚群。
