巢蛋白驱动的绿色荧光蛋白作为癌症小鼠模型中新生血管的成像标记物。

Nestin-driven green fluorescent protein as an imaging marker for nascent blood vessels in mouse models of cancer.

作者信息

Hoffman Robert M

机构信息

AntiCancer Inc and Department of Surgery, University of California, 7917, Ostrow Street, San Diego, CA 92111, USA.

出版信息

Methods Mol Biol. 2011;689:183-204. doi: 10.1007/978-1-60761-950-5_11.

DOI:10.1007/978-1-60761-950-5_11

PMID:21153793

Abstract

A transgenic mouse, in which the regulatory elements of the stem cell marker, nestin drive green fluorescent protein (ND-GFP), expresses GFP in nascent blood vessels. Red fluorescent protein (RFP)-expressing tumors transplanted to nestin-GFP mice enable specific visualization of nascent vessels in the growing tumors. The ND-GFP mouse was also utilized to develop a rapid in vivo/ex vivo fluorescent angiogenesis assay by implanting Gelfoam(®), a surgical sponge derived from pigskin, which was rapidly vascularized by fluorescent nascent blood vessels. Angiogenesis could be imaged and quantified when stimulated or inhibited by specific compounds in both tumors and Gelfoam(®). These fluorescent models can be used to study the early events of angiogenesis and to quantitatively determine efficacy of antiangiogenesis compounds.

摘要

一种转基因小鼠，其干细胞标志物巢蛋白的调控元件驱动绿色荧光蛋白（ND-GFP）表达，在新生血管中表达绿色荧光蛋白。将表达红色荧光蛋白（RFP）的肿瘤移植到巢蛋白-GFP小鼠体内，可使生长中的肿瘤内新生血管实现特异性可视化。ND-GFP小鼠还被用于开发一种快速的体内/体外荧光血管生成检测方法，通过植入明胶海绵（Gelfoam®）（一种源自猪皮的手术海绵）来实现，该海绵会迅速被荧光新生血管血管化。当在肿瘤和明胶海绵（Gelfoam®）中受到特定化合物刺激或抑制时，血管生成可以成像并定量。这些荧光模型可用于研究血管生成的早期事件，并定量确定抗血管生成化合物的疗效。