AntiCancer. Inc., 7917 Ostrow Street, San Diego, California 92111, USA.
Anticancer Res. 2013 Aug;33(8):3041-5.
Vessel anastomosis is important in tumor angiogenesis as well as for vascularization therapy for ischemia and other diseases. We report here the development of a color-coded imaging model that can visualize the anastomosis between blood vessels of red fluorescent protein (RFP)-expressing vessels in vascularized Gelfoam® previously transplanted into RFP transgenic mice and then re-transplanted into nestin-driven green fluorescent protein (ND-GFP) mice where nascent blood vessels express GFP. Gelfoam® was initially transplanted subcutaneously in the flank of transgenic RFP nude mice. Skin flaps were made at 14 days after transplantation of Gelfoam® to allow observation of vascularization of the Gelfoam® using confocal fluorescence imaging. The implanted Gelfoam® became highly vascularized with RFP vessels. Fourteen days after transplantation into RFP transgenic nude mice, the Gelfoam® was removed and re-transplanted into the subcutis on the flank of ND-GFP transgenic nude mice in which nascent blood vessels express GFP. Skin flaps were made and anastomosis between the GFP-expressing nascent blood vessels of ND-GFP transgenic nude mice and RFP blood vessels in the Gelfoam® was imaged 14 and 21 days after re-transplantation. The results presented in this report indicate a possible mechanism for tumor angiogenesis and suggest a new paradigm of therapeutic revascularization of ischemic organs requiring new blood vessels and in other diseases.
血管吻合在肿瘤血管生成以及缺血等疾病的血管化治疗中非常重要。我们在此报告一种彩色成像模型的开发，该模型可可视化先前移植到表达红色荧光蛋白（RFP）血管的血管化 Gelfoam®中的血管与新生血管表达 GFP 的巢蛋白驱动的绿色荧光蛋白（ND-GFP）小鼠中再移植的 RFP 转基因小鼠的血管之间的吻合。Gelfoam®最初被皮下移植到 RFP 转基因裸鼠的侧腹。在 Gelfoam®移植后 14 天制作皮肤瓣，允许使用共聚焦荧光成像观察 Gelfoam®的血管化情况。植入的 Gelfoam®变得高度血管化，有 RFP 血管。将 Gelfoam®移植到 RFP 转基因裸鼠后 14 天，将其取出并重新移植到 ND-GFP 转基因裸鼠的侧腹皮下，其中新生血管表达 GFP。制作皮肤瓣，并在再移植后 14 天和 21 天成像 ND-GFP 转基因裸鼠中 GFP 表达的新生血管与 Gelfoam®中 RFP 血管之间的吻合。本报告中的结果表明肿瘤血管生成的一种可能机制，并提出了一种新的缺血器官治疗性再血管化的范例，需要新的血管和其他疾病。
