Kato S, Ishii H, Aiso S, Yamashita S, Ito D, Tsuchiya M
Department of Internal Medicine, School of Medicine, Keio University, Tokyo, Japan.
Hepatology. 1990 Jul;12(1):66-9. doi: 10.1002/hep.1840120111.
The distribution of alcohol dehydrogenase in the hepatic acinus was examined by both histochemical and immunohistochemical approaches. The immunohistochemical method using anti-alcohol dehydrogenase antibody indicated zone 3 predominance of this enzyme in the hepatic acinus, whereas a conventional histochemical method showed slight zone 1 predominance. However, when the histochemical technique was improved by using 2% glutaraldehyde instead of formalin for fixation and by adding phenazine methosulfate (0.33 mmol/L) to the staining incubation mixture, this method also supported zone 3 predominance of alcohol dehydrogenase. Evidence for zone 3 distribution of alcohol dehydrogenase may be of value in elucidating the mechanism of zone 3 liver damage by alcohol.
通过组织化学和免疫组织化学方法研究了肝腺泡中乙醇脱氢酶的分布。使用抗乙醇脱氢酶抗体的免疫组织化学方法表明，该酶在肝腺泡中主要分布于3区，而传统组织化学方法显示其在1区略有优势。然而，当组织化学技术通过使用2%戊二醛而非福尔马林进行固定，并在染色孵育混合物中添加硫酸吩嗪甲酯（0.33 mmol/L）来改进时，该方法也支持乙醇脱氢酶在3区占优势。乙醇脱氢酶在3区分布的证据可能有助于阐明酒精导致3区肝损伤的机制。
