Salsano F, Maly I P, Sasse D
Anatomisches Institut, Basel, Switzerland.
Histochem J. 1990 Aug;22(8):395-400. doi: 10.1007/BF01003458.
Using microquantitative measurements of alcohol dehydrogenase activity in microdissected samples of liver tissue along the sinusoidal length, the intra-acinar distribution profiles were studied in seven groups of female rats at different times during 24 h with a light phase from 6:30 h to 18:30 h. The mean values of alcohol dehydrogenase activity showed a circadian rhythm with a minimum at 13.30 h and a maximum at 17.30 h (p less than 0.001). However, the intra-acinar gradients remained almost unchanged, indicating that increase and decrease in enzyme activity takes place simultaneously in all parts of the liver acinus. This observation, together with data from the literature, suggests that the circadian rhythm of alcohol dehydrogenase activity reflects variations in different liver cell constituents, rather than enzyme protein synthesis or proteolysis.
利用沿肝血窦长度对肝组织显微切割样本中的乙醇脱氢酶活性进行微量定量测量，在七组雌性大鼠中，于24小时内不同时间研究了腺泡内分布情况，光照期为6:30至18:30。乙醇脱氢酶活性的平均值呈现昼夜节律，在13:30时最低，在17:30时最高（p<0.001）。然而，腺泡内梯度几乎保持不变，表明酶活性的增加和降低在肝腺泡的所有部位同时发生。这一观察结果，连同文献数据表明，乙醇脱氢酶活性的昼夜节律反映了不同肝细胞成分的变化，而非酶蛋白的合成或蛋白水解。
