Kasturi S, Rudrammaji L M, Gowda T V
Department of Studies in Biochemistry, University of Mysore, Manasagangothri, India.
Immunology. 1990 Jun;70(2):175-80.
Polyclonal antibodies to a purified neurotoxic phospholipase A2 (PLA2), VRV PL-VIIIa, from Vipera russelli venom were raised in rabbits. Anti-PL-VIIIa-Ig (gamma-globulin fraction of rabbit antiserum injected with VRV PL-VIIIa) selectively neutralized the neurotoxicity of VRV PL-VIIIa, VRV PL-V, VRV PL-VI (neurotoxic PLA2 of V. russelli venom) and whole V. russelli venom without affecting their PLA2 activity, which clearly demonstrates that the catalytic site and the neurotoxic site (the site through which the PLA2 binds to the nervous system) are distinct on a PLA2 molecule. Anti-PL-VIIIa Ig did not have any effect on the oedema-inducing activity and indirect haemolytic activity of VRV PL-VIIIa, VRV PL-V and VRV PL-VI, which are attributed to the PLA2 activity of the peptide, but inhibited their anti-coagulant potency.
用来自罗素蝰蛇毒液中纯化的神经毒性磷脂酶A2（PLA2）——VRV PL-VIIIa，对家兔进行免疫以产生多克隆抗体。抗PL-VIIIa-Ig（注射VRV PL-VIIIa的兔抗血清的γ球蛋白组分）能选择性中和VRV PL-VIIIa、VRV PL-V、VRV PL-VI（罗素蝰蛇毒液的神经毒性PLA2）以及整个罗素蝰蛇毒液的神经毒性，而不影响它们的PLA2活性，这清楚地表明在PLA2分子上催化位点和神经毒性位点（PLA2与神经系统结合的位点）是不同的。抗PL-VIIIa Ig对VRV PL-VIIIa、VRV PL-V和VRV PL-VI的致水肿活性和间接溶血活性没有任何影响，这些活性归因于该肽的PLA2活性，但抑制了它们的抗凝血效力。
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