从罗素蝰蛇（Vipera russelli）毒液中纯化和鉴定一种主要的磷脂酶A2
Purification and characterization of a major phospholipase A2 from Russell's viper (Vipera russelli) venom.
作者信息
Kasturi S, Gowda T V
机构信息
Department of Studies in Biochemistry, Manasagangotri, University of Mysore, India.
出版信息
Toxicon. 1989;27(2):229-37. doi: 10.1016/0041-0101(89)90136-0.
A major phospholipase A2 (VRV PL-VIIIa) which constitutes 24% of the whole Vipera russelli venom was purified to homogeneity by CM-Sephadex C-25 column chromatography followed by gel filtration on Sephadex G-50. VRV PL-VIIIa is a basic protein with a molecular weight of 11,800 by SDS-PAGE. This enzyme contributes 45% of the total PLA2 activity of the venom, but it is least toxic compared to other purified basic PLA2 enzymes prepared from V. russelli venom. The LD50 value (i.p.) of VRV PL-VIIIa is 5.3 mg/kg body wt. It shows neurotoxic symptoms and damages vital organs such as lung, liver and kidney at LD50 doses. It induces myonecrosis when injected i.m. into the thigh muscle of mice and edema when injected into the foot pads.
一种占锯鳞蝰蛇毒总量24%的主要磷脂酶A2（VRV PL - VIIIa），通过CM - Sephadex C - 25柱色谱法，随后在Sephadex G - 50上进行凝胶过滤，被纯化至同质。通过SDS - PAGE分析，VRV PL - VIIIa是一种分子量为11,800的碱性蛋白质。这种酶占蛇毒总磷脂酶A2活性的45%，但与从锯鳞蝰蛇毒中制备的其他纯化碱性磷脂酶A2酶相比，其毒性最小。VRV PL - VIIIa的LD50值（腹腔注射）为5.3 mg/kg体重。它表现出神经毒性症状，在LD50剂量时会损害肺、肝和肾等重要器官。当肌肉注射到小鼠大腿肌肉中时，它会引起肌坏死，注射到脚垫中时会引起水肿。
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