[冷应激对鸡肺脏DNA氧化损伤的影响]
[The effect of cold stress on DNA oxidative damage of lung in chicken].
作者信息
Jia Hai-yan, Li Jin-min, Yu Qian, Wang Jun-jie, Li Shu
机构信息
College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China.
出版信息
Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2009 Aug;25(3):373-6.
AIM
To explore the influence of cold strss on DNA oxidative damage of lung in chicken.
METHODS
Took 15-day-old healthy chicks as the experimental object, carried on the cold stress (12 +/- 1 degrees C) to process. Detected the change of the MDA content, SOD and GSH-Px activity of the lung, and performed KCl-SDS precipitation method and fluorescence detection method to identify the influence of cold strss on DNA-protein crosslinks (DPC) and DNA-DNA crosslinks (DDC) of lung cell in different time.
RESULTS
The results were as follow: with the time lapsing during acute cold stress, MDA content gradually increased, the SOD and GSH-Px activity of the lung increased compared with their control group at each stress time point, and the lung cell DPC and DDC coefficient were all gradually increased with the time lapsing.
CONCLUSION
Cold stress could bring about destruction in the lung tissue oxidation-antioxidant balance, and causes the oxidation damage of DNA.
目的
探讨冷应激对雏鸡肺脏DNA氧化损伤的影响。
方法
以15日龄健康雏鸡为实验对象，进行冷应激（12±1℃）处理。检测肺脏丙二醛（MDA）含量、超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH-Px）活性的变化，并采用KCl-SDS沉淀法和荧光检测法，在不同时间点鉴定冷应激对肺细胞DNA-蛋白质交联（DPC）和DNA-DNA交联（DDC）的影响。
结果
结果如下：在急性冷应激过程中，随着时间的推移，MDA含量逐渐升高，各应激时间点肺脏SOD和GSH-Px活性较对照组均有所升高，且肺细胞DPC和DDC系数均随时间的推移逐渐升高。
结论
冷应激可破坏肺组织氧化-抗氧化平衡，导致DNA氧化损伤。
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