Institute of Natural Medicine, University of Toyama, Sugitani, Toyama, Japan.
J Pharm Pharmacol. 2011 Jan;63(1):111-9. doi: 10.1111/j.2042-7158.2010.01156.x. Epub 2010 Oct 11.
We have investigated the effects of Kangen-karyu, a Chinese prescription, on the lipid metabolism and oxidative stress in a type 2 diabetes model.
Male db/db mice were divided into three groups: control (vehicle), Kangen-karyu 100 or 200 mg/kg body weight/day orally administered mice. Age-matched non-diabetic m/m mice were used as a normal group.
The administration of Kangen-karyu reduced hyperglycaemia and hyperlipidaemia in db/db type 2 diabetic mice through a decline in the serum levels of glucose and lipids, and an improvement of lipoprotein profiles. The increased oxidative stress in db/db mice was attenuated by the administration of Kangen-karyu through inhibiting the generation of reactive oxygen species and lipid peroxidation. The enhanced hepatic triglyceride and total cholesterol levels of the db/db mice were significantly reduced by Kangen-karyu administration through down-regulation of sterol regulatory element-binding protein-1 and lipogenic enzymes in liver. Furthermore, the expressions of hepatic nuclear factor-kappa B (NF-κB) and cyclooxygenase-2 and inducible nitric oxide synthase protein levels were also augmented in db/db mice. However, Kangen-karyu reduced the expressions of these inflammatory proteins by inhibiting NF-κB activation in db/db type 2 diabetes.
This study suggests that Kangen-karyu may improve oxidative stress via the regulation of dyslipidaemia in type 2 diabetes.
我们研究了一款中药方剂还原丹对 2 型糖尿病模型的脂代谢和氧化应激的影响。
雄性 db/db 小鼠分为三组：对照组（溶剂）、还原丹 100 或 200mg/kg 体重/天灌胃组。年龄匹配的非糖尿病 m/m 小鼠作为正常组。
还原丹通过降低血清葡萄糖和脂质水平，改善脂蛋白谱，减少 2 型糖尿病 db/db 小鼠的高血糖和高脂血症。还原丹通过抑制活性氧和脂质过氧化的产生，减轻 db/db 小鼠的氧化应激。还原丹通过下调肝脏固醇调节元件结合蛋白-1 和脂肪生成酶，显著降低 db/db 小鼠的肝甘油三酯和总胆固醇水平。此外，db/db 小鼠的核因子-κB（NF-κB）和环氧合酶-2 和诱导型一氧化氮合酶蛋白水平的表达也增加。然而，还原丹通过抑制 NF-κB 激活，减少这些炎症蛋白的表达，从而改善 2 型糖尿病的氧化应激。
本研究表明，还原丹可能通过调节 2 型糖尿病的血脂异常来改善氧化应激。
