Department of Medicine, Rhode Island Hospital/Warren Alpert School of Medicine at Brown University, Providence, Rhode Island, USA.
Transfusion. 2011 Jul;51(7):1397-404. doi: 10.1111/j.1537-2995.2010.02984.x. Epub 2010 Dec 13.
During the transfusion of blood products, the transfer of allogeneic donor white blood cells (WBCs) can mediate adverse reactions in recipients. To minimize reactions, blood components are leukoreduced and/or exposed to gamma irradiation. Nucleic acid-targeted pathogen reduction technologies (PRTs) processes are well suited for WBC inactivation. The Mirasol PRT system (CaridianBCT Biotechnologies) uses riboflavin and ultraviolet light to reduce the active pathogen load and inactivate residual WBCs in blood products used for transfusion. The aim of this study was to compare the effect of PRT treatment to gamma irradiation.
WBCs were resuspended in 100% plasma or in plasma containing 65% platelet additive solution (SSP+). A single product was split into three aliquots: control, PRT treatment, or gamma irradiation. After treatment, peripheral blood mononuclear cells were isolated from products, and cell viability and functionality were assessed in limiting dilution assays (LDAs), by CD69 expression, by proliferation, and by cytokine accumulation after lipopolysaccharide addition.
PRT treatment and gamma irradiation reduced the ability of the T cells to proliferate by similar degrees as evidenced by a 6 log or greater reduction in the LDA and a lack of response to several stimuli. However, gamma-irradiated T cells were still capable of up regulating surface CD69, inducing a proliferative response in allogeneic responder cells, and producing levels of proinflammatory cytokines similar to those of untreated control cells, responses that PRT-treated T cells were incapable of mediating.
These in vitro results demonstrate that PRT treatment is more effective than gamma irradiation at abrogating selected WBC immune functions.
在输血过程中，同种异体供体白细胞（WBC）的转移会导致受者产生不良反应。为了最大限度地减少反应，血液成分需要经过白细胞减少处理和/或γ射线照射。核酸靶向病原体减少技术（PRT）处理非常适合 WBC 失活。Mirasol PRT 系统（CaridianBCT Biotechnologies）使用核黄素和紫外线减少活性病原体负荷，并在用于输血的血液制品中灭活残留的 WBC。本研究旨在比较 PRT 处理与γ射线照射的效果。
将 WBC 悬浮在 100%血浆或含有 65%血小板添加剂溶液（SSP+）的血浆中。将单个产品分为三份：对照、PRT 处理或γ射线照射。处理后，从产品中分离外周血单核细胞，并通过限制稀释分析（LDA）、CD69 表达、增殖和添加脂多糖后细胞因子积累来评估细胞活力和功能。
PRT 处理和γ射线照射以相似的程度降低了 T 细胞的增殖能力，证据是 LDA 减少了 6 个对数级以上，并且对多种刺激没有反应。然而，γ射线照射的 T 细胞仍然能够上调表面 CD69，诱导同种异体反应细胞的增殖反应，并产生与未处理对照细胞相似水平的促炎细胞因子，而 PRT 处理的 T 细胞无法介导这些反应。
这些体外结果表明，PRT 处理比γ射线照射更有效地消除选定的 WBC 免疫功能。
