National Center for Toxicological Research, Food and Drug Administration, Jefferson, Arkansas 72079-9502.
Mycologia. 2002 Jan-Feb;94(1):1-5.
The formation of conjugates from two antibacterial fluoroquinolone drugs, ciprofloxacin and norfloxacin, was observed in cultures of Trichoderma viride that had been grown in sucrose-peptone broth and extracted 16 d after dosing with the drugs. Both conjugates were purified by high-performance liquid chromatography and found to be optically active. They were identified by mass and proton nuclear magnetic resonance spectra as 4-hydroxy-3-oxo-4-vinylcyclopent-1-enyl ciprofloxacin and 4-hydroxy-3-oxo-4-vinylcyclopent-1-enyl norfloxacin. The transformation of veterinary fluoroquinolones in the presence of fungi may have ecological significance.
青霉属真菌培养物中观察到两种抗菌氟喹诺酮药物环丙沙星和诺氟沙星形成缀合物，这些真菌是在含有蔗糖和蛋白胨的肉汤中生长的，并在给药后 16 天提取。两种缀合物均通过高效液相色谱法进行纯化，并通过质谱和质子核磁共振光谱鉴定为 4-羟基-3-氧代-4-乙烯基环戊-1-烯基环丙沙星和 4-羟基-3-氧代-4-乙烯基环戊-1-烯基诺氟沙星。兽医氟喹诺酮类药物在真菌存在下的转化可能具有生态意义。
