Department of Botany, University of Georgia, Athens, Georgia 30602.
Mycologia. 2002 Mar-Apr;94(2):260-6.
The positions of all Woronin bodies in five germlings of Aspergillus nidulans prepared by plunge freezing and freeze substitution were determined by transmission electron microscopy. As expected, Woronin bodies were found near septa. High numbers of morphologically identical organelles were also found in apical regions. To verify that these organelles were authentic Woronin bodies, we used antibodies raised against the Neurospora crassa Woronin body matrix protein Hex1. Anti-Hex1 antibodies labeled Woronin bodies at septa and in apical regions of A. nidulans. In germlings that had not yet formed septa, at least fifty percent of Woronin bodies were found within 2.5 μm of the tip. In germ tubes that had formed septa, the total number of Woronin bodies remained the same, but only twenty percent were near the tip. Our results clearly establish that Woronin bodies are found in apical regions of Aspergillus germ tubes and suggest that Woronin bodies are transported from the apex to the more basal regions of the cell immediately before or during septation.
采用 plunge 冷冻和冷冻置换法制备的 5 个粗糙脉孢菌幼体的所有沃罗宁体的位置通过透射电子显微镜确定。正如预期的那样，沃罗宁体被发现靠近隔膜。在顶端区域还发现了大量形态相同的细胞器。为了验证这些细胞器是否是真正的沃罗宁体，我们使用了针对粗糙脉孢菌沃罗宁体基质蛋白 Hex1 的抗体。抗 Hex1 抗体在隔膜和 Aspergillus nidulans 的顶端区域标记了沃罗宁体。在尚未形成隔膜的幼体中，至少有 50%的沃罗宁体位于尖端 2.5μm 范围内。在已经形成隔膜的幼管中，沃罗宁体的总数保持不变，但只有 20%位于尖端附近。我们的结果清楚地表明，沃罗宁体存在于 Aspergillus 幼管的顶端区域，并表明沃罗宁体在隔膜形成之前或期间立即从顶端运输到细胞的更基底区域。
