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并且对于维持捕食线虫真菌的细胞壁完整性、营养生长、产孢、抗逆性和致病性是必需的。

and Are Necessary to Maintain Cell Wall Integrity, Vegetative Growth, Conidiation, Stress Resistance, and Pathogenicity in the Nematode-Trapping Fungus .

作者信息

Xie Meihua, Yang Jiangliu, Jiang Kexin, Bai Na, Zhu Meichen, Zhu Yingmei, Zhang Ke-Qin, Yang Jinkui

机构信息

State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, and Key Laboratory for Southwest Microbial Diversity of the Ministry of Education, Yunnan University, Kunming, China.

School of Resources, Environment and Chemistry, Chuxiong Normal University, Chuxiong, China.

出版信息

Front Microbiol. 2021 Jun 22;12:649582. doi: 10.3389/fmicb.2021.649582. eCollection 2021.

DOI:10.3389/fmicb.2021.649582
PMID:34239505
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8258383/
Abstract

The cell wall integrity (CWI) pathway is composed of three mitogen-activated protein kinases (MAPKs), Bck1, Mkk1/2, and Slt2, and is one of the main signaling pathways for fungal pathogenesis, cell wall synthesis, and integrity maintenance. In this study, we characterized orthologs of and in the nematode-trapping (NT) fungus by multiple phenotypic comparison, and the regulation of conidiation and cell wall synthesis was analyzed using real-time PCR (RT-PCR). Both Δ and Δ mutants showed severe defects in vegetative growth, cell nucleus number, and stress resistance. Both the mutants were unable to produce spores, and the transcription of several genes associated with sporulation and cell wall biosynthesis was markedly downregulated during the conidiation stage. Further, cell walls of the Δ and Δ mutants were severely damaged, and the Woronin body failed to respond to cellular damage. In particular, the mutants lost the ability to produce mycelial traps for nematode predation. Taken together, and play a conserved role in mycelial growth and development, CWI, conidiation, multi-stress tolerance, trap formation, and pathogenicity. We highlighted the role of and in regulating the Woronin body response to cellular damage and cell nucleus development in .

摘要

细胞壁完整性（CWI）途径由三种丝裂原活化蛋白激酶（MAPK），即Bck1、Mkk1/2和Slt2组成，是真菌致病、细胞壁合成和完整性维持的主要信号通路之一。在本研究中，我们通过多种表型比较对捕食线虫（NT）真菌中的 和 直系同源物进行了表征，并使用实时PCR（RT-PCR）分析了分生孢子形成和细胞壁合成的调控。Δ 和Δ 突变体在营养生长、细胞核数量和抗逆性方面均表现出严重缺陷。两个突变体均无法产生孢子，并且在分生孢子形成阶段，几个与孢子形成和细胞壁生物合成相关的基因的转录明显下调。此外，Δ 和Δ 突变体的细胞壁严重受损，沃罗宁体无法对细胞损伤做出反应。特别是，突变体失去了产生捕食线虫的菌丝陷阱的能力。综上所述， 和 在菌丝生长发育、CWI、分生孢子形成、多胁迫耐受性、陷阱形成和致病性方面发挥着保守作用。我们强调了 和 在调节沃罗宁体对细胞损伤的反应以及 细胞核发育中的作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/79d2/8258383/6fb5235bcbcd/fmicb-12-649582-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/79d2/8258383/28ceaf418bb0/fmicb-12-649582-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/79d2/8258383/f5df05a41dfe/fmicb-12-649582-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/79d2/8258383/a5deadc28e1b/fmicb-12-649582-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/79d2/8258383/58a1da96cac6/fmicb-12-649582-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/79d2/8258383/d014931b7588/fmicb-12-649582-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/79d2/8258383/bd2837bfc3d7/fmicb-12-649582-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/79d2/8258383/6fb5235bcbcd/fmicb-12-649582-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/79d2/8258383/28ceaf418bb0/fmicb-12-649582-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/79d2/8258383/f5df05a41dfe/fmicb-12-649582-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/79d2/8258383/a5deadc28e1b/fmicb-12-649582-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/79d2/8258383/58a1da96cac6/fmicb-12-649582-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/79d2/8258383/d014931b7588/fmicb-12-649582-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/79d2/8258383/bd2837bfc3d7/fmicb-12-649582-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/79d2/8258383/6fb5235bcbcd/fmicb-12-649582-g007.jpg

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