Department of Botany, PO Box 118526, University of Florida, Gainesville, Florida 32611-8526.
Mycologia. 2002 Mar-Apr;94(2):267-72.
We have approached the problem of hyphal tip growth by comparing the cell wall composition of elongating and non-elongating regions of the hyphae of Achlya bisexualis. To ensure that we could distinguish between elongating and non-elongating hyphae, light microscopic observations were used to determine the rates of elongation under growing and non-growing conditions. When elongation was measured in 10 min intervals it was found to consist of fluctuating periods of fast and slow growth rates, in the form of cycles. Even under our growing conditions, however, a very small number of hyphae in a colony are not elongating. SEM analysis revealed that elongating hyphae have tapered apices, whereas non-elongating hyphae have a rounded apex. The major matrix wall components, 1,3-β-glucans, were localized with an indirect immunogold technique specific for these polymers. This method resulted in their localization to all regions of both elongating and non-elongating hyphae, including the apex.
我们通过比较双相阿氏藻菌丝伸长和非伸长区域的细胞壁组成，研究了菌丝顶端生长的问题。为了确保能够区分伸长和非伸长的菌丝，我们采用了光学显微镜观察来确定在生长和非生长条件下的伸长率。当以 10 分钟为间隔测量伸长率时，发现它由快速和慢速生长率的波动周期组成，呈周期性变化。然而，即使在我们的生长条件下，菌落中仍有极少数菌丝不伸长。SEM 分析表明，伸长的菌丝具有锥形顶端，而不伸长的菌丝具有圆形顶端。主要基质壁成分 1,3-β-葡聚糖用针对这些聚合物的间接免疫胶体金技术进行了定位。该方法导致它们在包括顶端在内的所有伸长和非伸长菌丝区域的定位。
