Department of Botany, PO Box 118526, University of Florida, Gainesville, Florida 32611-8526.
Mycologia. 2002 Mar-Apr;94(2):273-9.
Cellulose has been localized in the hyphal wall of elongating and non-elongating hyphae of Achlya bisexualis using a direct enzyme-colloidal-gold method. A number of controls, including several different types of fixation, support the idea that this labeling is specific for cellulose. Both TEM and SEM were used and they gave similar results. The apical area of an elongating hypha lacks cellulose, but the same area of a non-elongating hypha contains cellulose. We have used specific culture media and light microscopic measurements to ensure that we could distinguish between elongating and non-elongating hyphae. The lack of cellulose at the apex of elongating hyphae seems to require a reevaluation of the current concepts of hyphal tip growth in Achlya and related genera. A major question now is to determine whether or not the lack of a microfibrillar component is a universal pattern among all organisms having tip growth.
使用直接酶-胶体金方法，我们将纤维素定位于双相阿氏藻的伸长和非伸长菌丝的菌丝壁中。许多对照实验，包括几种不同类型的固定方法，都支持这种标记是纤维素特异性的观点。我们同时使用了 TEM 和 SEM，它们给出了相似的结果。伸长菌丝的顶端区域不含纤维素，但同一非伸长菌丝的区域含有纤维素。我们使用了特定的培养基和光学显微镜测量来确保我们能够区分伸长和非伸长菌丝。在伸长菌丝的顶端缺乏纤维素，这似乎需要重新评估当前关于阿氏藻和相关属中菌丝顶端生长的概念。现在的一个主要问题是确定缺乏微纤维成分是否是所有具有顶端生长的生物体的普遍模式。
