Departamento de Biologia, Faculdade de Engenharia, Universidade Estadual Paulista "Júlio de Mesquita Filho" (UNESP), 15385-000, Ilha Solteira, São Paulo, Brazil.
Mycologia. 2002 May-Jun;94(3):450-60.
A polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method was developed to identify and differentiate genotypes of Rhizoctonia solani anastomosis group 3 subgroup PT (AG-3 PT), a fungal pathogen of potato. Polymorphic co-dominant single-locus PCR-RFLP markers were identified after sequencing of clones from a genomic library and digestion with restriction enzymes. Multilocus genotypes were determined by a combination of PCR product and digestion with a specific restriction enzyme for each of seven loci. A sample of 104 isolates from one commercial field in each of five counties in eastern North Carolina was analyzed, and evidence for high levels of gene flow between populations was revealed. When data were clone-corrected and samples pooled into one single North Carolina population, random associations of alleles were found for all loci or pairs of loci, indicating random mating. However, when all genotypes were analyzed, the observed genotypic diversity deviated from panmixia and alleles within and between loci were not randomly associated. These findings support a model of population structure for R. solani AG-3 PT on potato that includes both recombination and clonality.
建立了聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法，用于鉴定和区分马铃薯病原菌茄丝核菌 3 号吻合群 PT（AG-3 PT）的基因型。通过对基因组文库克隆进行测序并用限制性内切酶消化，鉴定出多态性共显性单基因座 PCR-RFLP 标记。通过组合每个 7 个基因座的 PCR 产物和特定的限制酶消化来确定多位点基因型。分析了来自北卡罗来纳州东部五个县一个商业田的 104 个分离株，结果表明种群之间存在高水平的基因流。当对数据进行克隆校正并将样本汇集到北卡罗来纳州的一个单一群体中时，发现所有基因座或基因座对的等位基因随机关联，表明随机交配。然而，当分析所有基因型时，观察到的基因型多样性偏离了泛群，并且基因座内和基因座之间的等位基因没有随机关联。这些发现支持了在马铃薯上茄丝核菌 AG-3 PT 的种群结构模型，该模型包括重组和克隆性。
