Department of Botany, University of Tennessee, Knoxville, Tennessee 37996.
Mycologia. 2002 May-Jun;94(3):461-71.
Artomyces pyxidatus (Auriscalpiaceae) is a lignicolous, coralloid basidiomycete found throughout temperate regions of the Northern Hemisphere. Previous studies established that populations from the eastern United States, Sweden, and China were conspecific based on mating compatibility and enzyme profiles. In this study, mating compatibility was extended to include collections from Russia, Costa Rica, Mexico, and Utah. The molecular diversity of A. pyxidatus was examined by DNA sequence and restriction site analyses of the nuclear ribosomal internally transcribed spacer region (ITS1-5.8S-ITS2). A phylogenetic analysis of twelve isolates based on ITS sequences revealed a broad geographical pattern in which Eurasian isolates comprise a sister clade to North American isolates. North American isolates appear to be further subdivided into northeastern and southwestern clades. A survey of 255 A. pyxidatus isolates using restriction enzymes revealed variable RFLP patterns that follow similar geographical patterns.
珊瑚状球盖菇（鸡油菌科）是一种木质腐生、珊瑚状的担子菌，分布于北半球温带地区。先前的研究基于交配相容性和酶谱，确定了来自美国东部、瑞典和中国的种群为同种。在这项研究中，交配相容性扩展到包括来自俄罗斯、哥斯达黎加、墨西哥和犹他州的标本。通过对核核糖体内转录间隔区（ITS1-5.8S-ITS2）的 DNA 序列和限制酶分析，研究了珊瑚状球盖菇的分子多样性。基于 ITS 序列的 12 个分离物的系统发育分析显示，在地理分布上，欧亚分离物与北美分离物构成姐妹群。北美分离物似乎进一步细分为东北和西南两个分支。对 255 株珊瑚状球盖菇分离物进行的限制酶调查显示，其可变的 RFLP 模式遵循类似的地理模式。
