Park Myung Soo, Lee Hyun, Oh Seung-Yoon, Jung Paul Eunil, Seok Soon Ja, Fong Jonathan J, Lim Young Woon
School of Biological Sciences, Seoul National University, Seoul, 151-747, Republic of Korea.
J Microbiol. 2014 Aug;52(8):631-8. doi: 10.1007/s12275-014-4168-z. Epub 2014 Jul 4.
Distinguishing individual Russula species can be very difficult due to extensive phenotypic plasticity and obscure morphological and anatomical discontinuities. In this study, we use the internal transcribed spacer (ITS) and 28S nuclear ribosomal large subunit (LSU) markers to identify and study the genetic diversity of species in the Russula subgenus Compacta in Korea. We focus on two morphologically similar species that are often misidentified for each other: R. nigricans and R. subnigricans. Based on molecular phylogenetic analyses, we identify three subgroups of R. nigricans, with two from Asia and one from Europe/North America. Surprisingly, we find Korean R. subnigricans are more closely related to R. eccentrica from North America than the type specimen of R. subnigricans from Japan. These molecular data, along with habitat data, reveal that Korean R. subnigricans had previously been misclassified and should now be recognized as R. eccentrica. Both ITS and LSU exhibit high interspecific and low intraspecific variation for R. eccentrica, R. nigricans, and R. subnigricans. These markers provide enough resolutional power to differentiate these species and uncover phylogeographic structure, and will be powerful tools for future ecological studies of Russula.
由于广泛的表型可塑性以及模糊的形态和解剖学间断性，区分红菇属的各个物种可能非常困难。在本研究中，我们使用内部转录间隔区（ITS）和28S核糖体大亚基（LSU）标记来鉴定和研究韩国致密红菇亚属物种的遗传多样性。我们聚焦于两个形态相似且常被彼此误认的物种：黑红菇和亚黑红菇。基于分子系统发育分析，我们鉴定出黑红菇的三个亚组，其中两个来自亚洲，一个来自欧洲/北美。令人惊讶的是，我们发现韩国的亚黑红菇与来自北美的偏心红菇的亲缘关系比来自日本的亚黑红菇模式标本更近。这些分子数据以及栖息地数据表明，韩国的亚黑红菇此前被错误分类，现在应被认定为偏心红菇。ITS和LSU在偏心红菇、黑红菇和亚黑红菇中均表现出高种间差异和低种内差异。这些标记提供了足够的分辨能力来区分这些物种并揭示系统地理学结构，将成为未来红菇生态研究的有力工具。
