Real Jardín Botánico, C.S.I.C., Plaza de Murillo 2, 28014 Madrid, Spain.
Mycologia. 2003 May-Jun;95(3):474-9. doi: 10.1080/15572536.2004.11833092.
Four new primers were designed, based on comparison of Physarum polycephalum sequences retrieved from Genbank (primers PHYS-5 and PHYS-4) and our own sequences (primers PHYS-3 and PHYS-2), to amplify the ITS regions of rDNA, including the 5.8S gene segment from Lamproderma species. Sequencing analysis shows that Lamproderma contains ITS1-5.8S-ITS2 regions of approximately 900 bp, which is similar in size to most eukaryotes. However, the corresponding region in another common myxomycete, Fuligo septica, is more than 2000 bp due to the presence of large direct-repeat motifs in ITS1. Myxomycete rDNA ITS regions are interesting both as phylogenetic markers in taxonomic studies and as model sequences for molecular evolution.
基于对从 Genbank 检索到的多头绒泡菌（Physarum polycephalum）序列（引物 PHYS-5 和 PHYS-4）和我们自己的序列（引物 PHYS-3 和 PHYS-2）的比较，设计了四个新引物，以扩增 rDNA 的 ITS 区，包括 Lamproderma 物种的 5.8S 基因片段。测序分析表明，Lamproderma 含有约 900bp 的 ITS1-5.8S-ITS2 区，大小与大多数真核生物相似。然而，在另一种常见的粘菌—— Septica 中，由于 ITS1 中存在大的直接重复序列，相应的区域超过 2000bp。粘菌类 rDNA ITS 区作为分类研究中的系统发育标记和分子进化的模型序列都很有趣。
