Department of Genetics, University of North Carolina, Chapel Hill, North Carolina 27599-7264, USA.
Physiol Genomics. 2011 Feb 24;43(4):199-212. doi: 10.1152/physiolgenomics.00217.2010. Epub 2010 Dec 14.
The regulation of body weight and composition is complex, simultaneously affected by genetic architecture, the environment, and their interactions. We sought to analyze the complex phenotypic relationships between voluntary exercise, food consumption, and changes in body weight and composition and simultaneously localize quantitative trait loci (QTL) controlling these traits. A large (n = 815) murine advanced intercross line (G(4)) was created from a reciprocal cross between a high-running line and the inbred strain C57BL/6J. Body weight and composition (% fat, % lean) were measured at 4, 6, and 8 wk of age. After measurements at 8 wk of age, mice were given access to running wheels, during which food consumption was quantified and after which body weight and composition were assessed to evaluate exercise-induced changes. Phenotypic correlations indicated that the relationship between exercise and overall change in weight and adiposity depended on body composition before the initiation of exercise. Interval mapping revealed QTL for body weight, % fat, and % lean at 4, 6, and 8 wk of age. Furthermore, QTL were observed for food consumption and changes in weight, % fat, and % lean in response to short-term exercise. Here we provide some clarity for the relationship between weight loss, reduction in adiposity, food consumption, and exercise. Simultaneously, we reinforce the genetic basis for body weight and composition with some independent loci controlling growth at different ages. Finally, we present unique QTL providing insight regarding variation in weight loss and reduction in adiposity in response to exercise.
体重和成分的调节是复杂的，同时受到遗传结构、环境及其相互作用的影响。我们试图分析自愿运动、食物消耗与体重和成分变化之间复杂的表型关系，并同时定位控制这些特征的数量性状基因座 (QTL)。从一个高跑线和近交系 C57BL/6J 的回交中创建了一个大型（n = 815）鼠类先进的互交系（G(4)）。体重和成分（%脂肪，%瘦肉）在 4、6 和 8 周龄时测量。在 8 周龄时测量后，小鼠可以使用跑步轮，在此期间量化食物消耗，然后评估体重和成分以评估运动引起的变化。表型相关性表明，运动与体重和肥胖总体变化之间的关系取决于运动前的身体成分。区间作图显示了 4、6 和 8 周龄时体重、%脂肪和%瘦肉的 QTL。此外，还观察到了与短期运动相关的食物消耗和体重、%脂肪和%瘦肉变化的 QTL。在这里，我们为体重减轻、体脂减少、食物消耗和运动之间的关系提供了一些清晰的认识。同时，我们用一些独立的控制不同年龄生长的基因座来强化体重和成分的遗传基础。最后，我们提出了一些独特的 QTL，为运动减肥和体脂减少的变化提供了一些见解。
