Zhao Liangcai, Liu Xia, Xie Liyun, Gao Hongchang, Lin Donghai
Shanghai Institute of Materia Medica, The Chinese Academy of Sciences, Shanghai, P. R. China.
Anal Sci. 2010;26(12):1277-82. doi: 10.2116/analsci.26.1277.
Diabetes mellitus is a complex metabolic disorder characterized by chronic hyperglycemia, hypoinsulinemia, and ketosis. To access the biochemical process of diabetes, we applied quantitative (1)H NMR-based metabonomics to analyze urine, serum, and liver extracts from streptozotocin-induced diabetic rats. Principle component analysis (PCA) of (1)H NMR spectra disclosed metabolic pattern differences between diabetic and control rats, and identified the related metabolic changes. The PCA scores plot demonstrated that the diabetic group could be distinguished from the control group, indicating that the metabolic characteristics of the two groups were markedly different. Our work reveals the accumulation of triglycerides, fatty acids and acetoacetate in diabetic rats, and may provide an efficient, convenient way for evaluating the pathological state and biochemical process of diabetes mellitus.
糖尿病是一种复杂的代谢紊乱疾病，其特征为慢性高血糖、低胰岛素血症和酮症。为了探究糖尿病的生化过程，我们应用基于定量氢核磁共振（1H NMR）的代谢组学方法，分析链脲佐菌素诱导的糖尿病大鼠的尿液、血清和肝脏提取物。对1H NMR光谱进行主成分分析（PCA），揭示了糖尿病大鼠和对照大鼠之间的代谢模式差异，并确定了相关的代谢变化。PCA得分图表明糖尿病组与对照组能够区分开来，这表明两组的代谢特征明显不同。我们的研究揭示了糖尿病大鼠中甘油三酯、脂肪酸和乙酰乙酸的积累，并且可能为评估糖尿病的病理状态和生化过程提供一种高效、便捷的方法。
